PAD6 recombinant protein

PAD6 recombinant protein

Cat. Num. AAA515212

• Gene Names: PADI6; hPADVI
• Applications: Western Blot
• Synonyms: PAD6; PAD6 recombinant protein; PADI6

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCI, pH 7.5, 50mM NaCI, 10 mM glutathione,0.1 mM EDTA, 0.25mM DTT, 0.01 PMSF, 25% glycerol.
• Concentration: 0.1ug/ul (varies by lot)
• Sequence Length: 2346

• Applicable Applications for PAD6 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: N-terminal GST
• Source Note: Recombinant full-length human PAD6 was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; ; Stability: 1 year at -70°C from date of shipment.

Test Data

(The purity of PAD6 was determined to be >95% by densitometry. Approx. MW 100 kDa .)

Related Product Information for PAD6 recombinant protein

Recombinant full-length human PAD6 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_207421.
Scientific Background PAD6 or PADI6 is a member of the peptidylarginine deiminases which convert arginine residues to citru lline residues in the presence of calcium ions. The PAD family members are thought to be involved in multiple sclerosis and rheumatoid arthritis pathophysiology, and they play a role in epidermis homeostasis 1). PAD6 is essent ial for formation of a novel oocyte-restricted fibrous structure, the cytoplasmic lattices CPLs). PAD6/CPL superstru cture plays a key role in regulating microtubule-mediated organelle positioning and movement (2).

Product Categories/Family for PAD6 recombinant protein

Arginine Deiminases Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 117414157
• NCBI GeneID: 353238
• NCBI Accession #: NP_997304.3
• NCBI GenBank Nucleotide #: NM_207421
• UniProt Accession #: Q6TGC4; Q330K5; Q70SX3
• Molecular Weight: 100 kDa
• NCBI Official Full Name: Homo sapiens peptidyl arginine deiminase, type VI (PADI6), mRNA
• NCBI Official Synonym Full Names: peptidyl arginine deiminase, type VI
• NCBI Official Symbol: PADI6
• NCBI Official Synonym Symbols: hPADVI
• NCBI Protein Information: protein-arginine deiminase type-6; peptidylarginine deiminase type 6; peptidyl arginine deiminase-like protein
• UniProt Protein Name: Protein-arginine deiminase type-6
• UniProt Gene Name: PADI6
• UniProt Synonym Gene Names: hPADVI
• UniProt Entry Name: PADI6_HUMAN

NCBI Description

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. This protein may play a role in cytoskeletal reorganization in the egg and in early embryo development. [provided by RefSeq, Sep 2012]

Uniprot Description

Function: Catalyzes the deimination of arginine residues of proteins. May be involved in cytoskeletal reorganization in the egg and early embryo

By similarity.

Catalytic activity: Protein L-arginine + H2O = protein L-citrulline + NH3.

Cofactor: Calcium

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity.

Tissue specificity: Ovary, testis and peripheral blood leukocytes. Ref.1

Sequence similarities: Belongs to the protein arginine deiminase family.