Mouse Microtubule Associated 2 (MAP2) Monoclonal Antibody | anti-MAP2 antibody

Microtubule Associated Protein 2 (MAP2)

Cat. Num. AAA502234

• Gene Names: MAP2; MAP2A; MAP2B; MAP2C
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Protein G purified culture supernatant
• Synonyms: Microtubule Associated 2 (MAP2); Monoclonal Antibody; Microtubule Associated Protein 2 (MAP2); DKFZp686I2148 antibody; MAP 2 antibody; MAP dendrite specific antibody; MAP-2 antibody; MAP2 antibody; MAP2A antibody; MAP2B antibody; MAP2C antibody; Microtubule associated protein 2 antibody; Microtubule-associated protein 2 antibody; MTAP2_HUMAN antibody; anti-MAP2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4H5
• Purity/Purification: Protein G purified culture supernatant
• Form/Format: 100ul in PBS + 50% glycerol + 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

• Applicable Applications for anti-MAP2 antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000; IHC: 1:1000; ICC: 1:1000; Our MAP2 mouse monoclonal antibody is Protein G purified. It is an excellent dendritic marker and is optimized in WB, IHC, IF, and ICC.
• Species Positively Tested: Bovine, Human, Mouse, Rat
• Immunogen Species: Bovine
• Immunogen Information: Purified bovine protein
• Antigen: The antigen is purified bovine MAP2. The antibody is protein G purified from culture supernatant.
• Preparation and Storage: Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20 degree C.; Shipping: Blue Ice; Country of Origin: USA

Immunohistochemistry (IHC)

(Immunostaining of rat hippocampus showing specific labeling of neuronal dendrites and perikarya with Anti-Microtubule Associated Protein 2 in green, FOX3 in red and nuclei in blue.)

Western Blot (WB)

(Western blot of mouse cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.)

Related Product Information for anti-MAP2 antibody

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).

Product Categories/Family for anti-MAP2 antibody

Cell Markers; Neuronal Markers

NCBI and Uniprot Product Information

• NCBI GI #: 87578396
• NCBI GeneID: 4133
• NCBI Accession #: NP_002365.3
• NCBI GenBank Nucleotide #: NM_002374.3
• UniProt Accession #: P11137; Q17S04; Q8IUX2; Q99975; Q99976
• Molecular Weight: 199,526 Da
• NCBI Official Full Name: microtubule-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: microtubule-associated protein 2
• NCBI Official Symbol: MAP2
• NCBI Official Synonym Symbols: MAP2A; MAP2B; MAP2C
• NCBI Protein Information: microtubule-associated protein 2; MAP-2
• UniProt Protein Name: Microtubule-associated protein 2
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP2
• UniProt Synonym Gene Names: MAP-2
• UniProt Entry Name: MTAP2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34-q35

Cellular Component: microtubule; microtubule associated complex; cell soma; cytoplasm; nucleolus; dendritic shaft

Molecular Function: calmodulin binding; protein binding; microtubule binding; structural molecule activity

Biological Process: axonogenesis; dendrite morphogenesis; microtubule bundle formation; neurite development; central nervous system neuron development