Typical Testing Data/Standard Curve (for reference only)
Renin-1 ELISA Kit | Ren1 elisa kit
Renin-1 (Mouse) ELISA
Gene Names
Ren1; Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352
Synonyms
Renin-1; Renin-1 (Mouse) ELISA; Ren1 elisa kit
Sequence Length
402
Application Notes
90 min. incubation (37 degree C) + 1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 3 hours, 30 min. total incubation time.
Samples
Mouse serum, plasma or cell culture supernates
Assay Type
Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
<10 pg/ml
Preparation and Storage
Store at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Ren1 elisa kit
Intended Uses: Enzyme-linked immune-sorbent assay (ELISA) for the determination of Renin-1 in mouse serum, plasma or cell culture supernates.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Renin-1 monoclonal antibody is pre-coated onto 96-well plates. Biotin conjugated anti-Renin-1 polyclonal antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the Renin-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Renin-1 can be calculated.
Background: The secretion of renin from granules stored in renal juxtaglomerular cells plays a key role in blood pressure homeostasis. The synthesis and release of renin and the extent of granulation is regulated by several mechanisms including signaling from the macula densa, neuronal input, and blood pressure. Clark AF et al study indicate that expression of the Ren-1(d) gene is a prerequisite for the formation of storage granules, even though the related protein renin-2 is present in these mice, suggesting that renin-1(d) and renin-2 are secreted by distinct pathways in vivo. The synthesis of renin and other biologically active polypeptides in the granular convoluted tubule cells of the mouse submandibular gland (SMG) is regulated by androgen and thyroid hormones. The androgen and thyroid hormones influence levels of renin-1 in mouse SMG primarily by regulating the amount of renin-1 mRNA available for translation.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Renin-1 monoclonal antibody is pre-coated onto 96-well plates. Biotin conjugated anti-Renin-1 polyclonal antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the Renin-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Renin-1 can be calculated.
Background: The secretion of renin from granules stored in renal juxtaglomerular cells plays a key role in blood pressure homeostasis. The synthesis and release of renin and the extent of granulation is regulated by several mechanisms including signaling from the macula densa, neuronal input, and blood pressure. Clark AF et al study indicate that expression of the Ren-1(d) gene is a prerequisite for the formation of storage granules, even though the related protein renin-2 is present in these mice, suggesting that renin-1(d) and renin-2 are secreted by distinct pathways in vivo. The synthesis of renin and other biologically active polypeptides in the granular convoluted tubule cells of the mouse submandibular gland (SMG) is regulated by androgen and thyroid hormones. The androgen and thyroid hormones influence levels of renin-1 in mouse SMG primarily by regulating the amount of renin-1 mRNA available for translation.
Product Categories/Family for Ren1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,343 Da
NCBI Official Full Name
renin-1
NCBI Official Synonym Full Names
renin 1 structural
NCBI Official Symbol
Ren1
NCBI Official Synonym Symbols
Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352
NCBI Protein Information
renin-1
UniProt Protein Name
Renin-1
Protein Family
UniProt Gene Name
Ren1
UniProt Synonym Gene Names
Ren; Ren-1
Uniprot Description
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Research Articles on Ren1
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Product Notes
The Ren1 ren1 (Catalog #AAA490247) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 90 min. incubation (37 degree C) + 1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 3 hours, 30 min. total incubation time. Researchers should empirically determine the suitability of the Ren1 ren1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Renin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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