Protein-C ELISA Kit | PROC elisa kit
Protein-C ELISA
Gene Names
PROC; PC; APC; PROC1; THPH3; THPH4
Synonyms
Protein-C; Protein-C ELISA; PROC elisa kit
Specificity
The microtiter plates are coated with a capture antibody specific for human Protein-C. In accordance with laboratory recommendations, a sample was considered deficient in the analyte when less than 70% of the normal value was measured.
Sequence Length
84
Application Notes
30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time.
Samples
Citrated human plasma
Preparation and Storage
Store at 2-8 degree C
Related Product Information for PROC elisa kit
Intended Uses: This Protein-C ELISA is a solid phase enzyme immunoassay for the determination of Protein-C in citrated human plasma.
Principle of the Assay: This test is using microplates coated with a capture antibody specific for human Protein-C. Diluted plasma samples (1:51) are incubated in the wells allowing Protein C present in the plasma to bind to the antibody. The unbound fraction is removed by washing. Afterwards anti-human Protein- C detection antibody conjugated to horseradish peroxidase (conjugate) is incubated and reacts with the antigen- antibody complex on the microwell surface. Following incubation, unbound conjugate is washed off. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The rate of color formation from the chromogen is measured in optical density units with a spectrophotometer at 450 nm. Using a curve prepared from the Reference Plasma provided with the kit, the Protein C antigen relative percent concentration in the plasma samples can be determined.
Background: Protein C is a vitamin K-dependent inactive zymogen of a serine protease that is mainly synthesized by hepatocytes in the liver. It has a molecular weight of 62 kDa and is present at a concentration of 4 ug/ml in the plasma. Activated Protein C (aPC) is a key component of the Protein C anticoagulant system that is activated by the binding of thrombin to the endothelial transmembrane receptor thrombomodulin. The complex of thrombin and thrombomodulin activates Protein C and the activated Protein C in turn forms a complex with its cofactor Protein S that has a high affinity to phospholipid membranes. This is of physiological importance since aPC inactivates preferentially the membrane-bound coagulation factors Va and VIIIa. Additionally, activated Protein C possesses profibrinolytic activity by inhibiting plasmin activator inhibitor-1 (PAI-1). Protein C deficiency may be inherited or acquired and is associated with a variably increased risk of thrombosis. The prevalence of Protein C deficiency has been estimated to be up to one case per 300 in the general population. Nearly 50-80 % of individuals with inherited Protein C deficiency will experience a thrombotic event before the age of 30-45. Individuals with a homozygous Protein C deficiency may suffer from neonatal purpura fulminans or massive venous thrombosis. Acquired Protein C deficiency is often associated with liver disease, surgery, oral anticoagulant therapy, antiphospholipid syndrome, etc. Protein C deficiency is classified in two states. Type I deficiency is a reduction in the level of Protein C. Type II deficiency is characterized by a reduced Protein C activity, with normal antigen level.
Principle of the Assay: This test is using microplates coated with a capture antibody specific for human Protein-C. Diluted plasma samples (1:51) are incubated in the wells allowing Protein C present in the plasma to bind to the antibody. The unbound fraction is removed by washing. Afterwards anti-human Protein- C detection antibody conjugated to horseradish peroxidase (conjugate) is incubated and reacts with the antigen- antibody complex on the microwell surface. Following incubation, unbound conjugate is washed off. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The rate of color formation from the chromogen is measured in optical density units with a spectrophotometer at 450 nm. Using a curve prepared from the Reference Plasma provided with the kit, the Protein C antigen relative percent concentration in the plasma samples can be determined.
Background: Protein C is a vitamin K-dependent inactive zymogen of a serine protease that is mainly synthesized by hepatocytes in the liver. It has a molecular weight of 62 kDa and is present at a concentration of 4 ug/ml in the plasma. Activated Protein C (aPC) is a key component of the Protein C anticoagulant system that is activated by the binding of thrombin to the endothelial transmembrane receptor thrombomodulin. The complex of thrombin and thrombomodulin activates Protein C and the activated Protein C in turn forms a complex with its cofactor Protein S that has a high affinity to phospholipid membranes. This is of physiological importance since aPC inactivates preferentially the membrane-bound coagulation factors Va and VIIIa. Additionally, activated Protein C possesses profibrinolytic activity by inhibiting plasmin activator inhibitor-1 (PAI-1). Protein C deficiency may be inherited or acquired and is associated with a variably increased risk of thrombosis. The prevalence of Protein C deficiency has been estimated to be up to one case per 300 in the general population. Nearly 50-80 % of individuals with inherited Protein C deficiency will experience a thrombotic event before the age of 30-45. Individuals with a homozygous Protein C deficiency may suffer from neonatal purpura fulminans or massive venous thrombosis. Acquired Protein C deficiency is often associated with liver disease, surgery, oral anticoagulant therapy, antiphospholipid syndrome, etc. Protein C deficiency is classified in two states. Type I deficiency is a reduction in the level of Protein C. Type II deficiency is characterized by a reduced Protein C activity, with normal antigen level.
Product Categories/Family for PROC elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,556 Da
NCBI Official Full Name
protein C, partial
NCBI Official Synonym Full Names
protein C, inactivator of coagulation factors Va and VIIIa
NCBI Official Symbol
PROC
NCBI Official Synonym Symbols
PC; APC; PROC1; THPH3; THPH4
NCBI Protein Information
vitamin K-dependent protein C
UniProt Protein Name
Vitamin K-dependent protein C
Protein Family
UniProt Gene Name
PROC
NCBI Description
This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]
Uniprot Description
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
Research Articles on PROC
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Product Notes
The PROC proc (Catalog #AAA490242) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time. Researchers should empirically determine the suitability of the PROC proc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein-C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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