Human anti-Human CD80 Monoclonal Antibody | anti-CD80 antibody

Anti-CD80 [16C10]

Cat. Num. AAA488796

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Reactivity: Human
• Applications: ELISA
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: CD80; Monoclonal Antibody; Anti-CD80 [16C10]; B7.1; CD28L; CD28LG; B7-1; B7; BB1; B7/BB1;T-lymphocyte activation antigen CD80; Activation B7-1 antigen; CTLA-4 counter-receptor B7.1; CTLA4L; CTLA-4L; anti-CD80 antibody

• Host: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Human IgG1, lambda
• Clone Number: 16C10
• Specificity: This antibody binds human CD80 and inhibits the B7.1:CD28 pathway.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD80 antibody: Blocking (BL), ELISA (EIA), Therapeutics
• Application Notes: This antibody can be used for identification of human B7.1 antigen and is capable of inhibiting the binding of B7.1 to CD28, however it does not block the binding of B7.1 to CTLA-4. This antibody further inhibits the production of IL-2. Blocking of the primary activation signal between CD28 and B7.1 (CD80) with these antibodies while allowing the unimpaired or coincident interaction of CTLA-4 and B7.1 represents a combined antagonistic effect on positive co-stimulation with an agonistic effect on negative signaling. The binding of the antibody to soluble B7.1 was tested using ELISA. This antibody tested positive for binding to human C1q protein. This antibody is reported to cause keratinocyte hyperproliferation and decrease in psoriasis by inhibiting the B7:CD28 pathway. This antibody is further reported to be useful for the treatment of intestinal inflammation and rheumatoid arthritis.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody 16C10 (MBS488796) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 16C10 (MBS488797) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488797, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometry using the anti-CD80 antibody 16C10 (MBS488796). Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG1 version of 16C10 (MBS488797, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Related Product Information for anti-CD80 antibody

Recombinant monoclonal antibody to CD80. Recombinant Platform with variable regions (i.e. specificity) from the heterohybridoma 16C10.
This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen.

NCBI and Uniprot Product Information

• NCBI GI #: 461606
• NCBI GeneID: 941
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• Molecular Weight: 18,761 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.