CD40 recombinant antibody

Anti-CD40 [5D12]

Cat. Num. AAA488353

• Gene Names: CD40; p50; Bp50; CDW40; TNFRSF5
• Reactivity: Cynomolgus, Marmoset Monkey, Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
• Purity: Protein A Affinity Purified
• Synonyms: CD40; Monoclonal Recombinant Antibody; Anti-CD40 [5D12]; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40 recombinant antibody

• Reactivity: Cynomolgus, Marmoset Monkey, Human
• Clonality: Monoclonal
• Isotype: Human IgG4, Kappa
• Clone Number: 5D12
• Specificity: ch5D12 binds specifically to recombinant and native human CD40 ectodomain and has been shown to also bind CD40 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (Hager et al, 2003) (D1 domain is crucial for CD40L binding). Binding characteristics are very similar between humanized ch5D12 and mu5D12. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis.
• Purity/Purification: Protein A Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 277

• Applicable Applications for CD40 recombinant antibody: Block (BL), ELISA (EIA), Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: ch5D12 is an antagonist of the CD40-CD40L pathway. ch5D12 binds to B cells and monocytes, but not to peripheral blood CD4+ and CD8+ cells. ch5D12 was tested in vivo using a marmoset EAE (experimental autoimmune encephalomyelitis) model (recombinant myelin oligodendrocyte glycoprotein (MOG) was used to induce EAE)-model for human multiple sclerosis. ch5D12 was able to prevent development of the symptoms of the disease during the treatment period, reduce the anti-MOG antibody response and reduce the formation of inflammatory lesions in the CNS. ch5D12 binding and specificity can be detected and measured by FC, SPR, ELISA and IP, and CD40+ cells can be stained by the antibody in IHC.
• Immunogen: Anti-CD40 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Labeled Substance: None
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Related Product Information for CD40 recombinant antibody

Recombinant monoclonal antibody to CD40. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5D12.
Anti-CD40 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.

Product Categories/Family for CD40 recombinant antibody

Chronic Inflammatory Autoimmune Diseases; Transplantation Rejection; EAE; Experimental Autoimmune Encephalomyelitis; Multiple Sclerosis; Inflammation; Humanized Antibody

References

Boon et al. Preclinical assessment of anti-CD40 Mab 5D12 in cynomolgus monkeys Toxicology. 2002 May 15; 174(1):53-65. PMID:11972992

NCBI and Uniprot Product Information

• NCBI GI #: 4507581
• NCBI GeneID: 958
• NCBI Accession #: NP_001241.1
• NCBI GenBank Nucleotide #: NM_001250.5
• UniProt Accession #: P25942; Q53GN5; Q5JY15; Q5U007; Q7M4Q8; Q86YK5; Q9BYU0; E1P5S9
• Molecular Weight: 22,259 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 5 isoform 1
• NCBI Official Synonym Full Names: CD40 molecule
• NCBI Official Symbol: CD40
• NCBI Official Synonym Symbols: p50; Bp50; CDW40; TNFRSF5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• Protein Family: CD40 ligand
• UniProt Gene Name: CD40
• UniProt Synonym Gene Names: TNFRSF5

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.