Porcine Mitochondrial Ribosomal Protein L17 ELISA Kit | MRPL17 elisa kit

Porcine Mitochondrial Ribosomal Protein L17 ELISA Kit

Cat. Num. AAA745692

• Gene Names: MRPL17; LIP2; L17mt; RPL17L; RPML26; MRP-L17; MRP-L26
• Reactivity: Porcine
• Synonyms: Mitochondrial Ribosomal Protein L17; Porcine Mitochondrial Ribosomal Protein L17 ELISA Kit; MRPL17 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This MRPL17 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine MRPL17. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MRPL17 elisa kit

Principle of the assay: MRPL17 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MRPL17 antibody and an MRPL17-HRP conjugate. The assay sample and buffer are incubated together with MRPL17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRPL17 concentration since MRPL17 from samples and MRPL17-HRP conjugate compete for the anti-MRPL17 antibody binding site. Since the number of sites is limited, as more sites are occupied by MRPL17 from the sample, fewer sites are left to bind MRPL17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRPL17 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MRPL17 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 48146471
• NCBI GeneID: 63875
• UniProt Accession #: Q9NRX2; Q6IAH8; Q96Q53; Q9C066; D3DQU3
• Molecular Weight: 20,050 Da
• NCBI Official Full Name: MRPL17
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L17
• NCBI Official Symbol: MRPL17
• NCBI Official Synonym Symbols: LIP2; L17mt; RPL17L; RPML26; MRP-L17; MRP-L26
• NCBI Protein Information: 39S ribosomal protein L17, mitochondrial; LYST-interacting protein 2; LYST-interacting protein LIP2
• UniProt Protein Name: 39S ribosomal protein L17, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL17
• UniProt Synonym Gene Names: LIP2; L17mt; MRP-L17
• UniProt Entry Name: RM17_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL17: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; Translation; Ribosomal

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit

Molecular Function: protein domain specific binding; structural constituent of ribosome

Biological Process: mitochondrial genome maintenance; mitochondrial translation; organelle organization and biogenesis