Fc gamma receptor III Recombinant Antibody
Anti-Fc gamma receptor III [3G8]
Reactivity
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
Fc gamma receptor III; Monoclonal Recombinant Antibody; Anti-Fc gamma receptor III [3G8]; CD16; low affinity IgG receptor III; FcgammaRIII; low affinity FcgammaRIII; Fc gamma receptor III recombinant antibody
Reactivity
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Clone Number
3G8
Specificity
3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity).
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
85
Applicable Applications for Fc gamma receptor III recombinant antibody
Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR), Depletion, Immunoprecipitation (IP), Immunohistochemistry (IHC), Block (BL)
Application Notes
3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies.
Immunogen
3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.
Buffer Composition
PBS only with 0.02% Proclin 300.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for Fc gamma receptor III recombinant antibody
Recombinant monoclonal antibody to Fc gamma receptor III. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3G8.
3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.
3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.
Product Categories/Family for Fc gamma receptor III recombinant antibody
References
Bruenke et al. A recombinant bispecific single-chain Fv antibody against HLA class II and FcgammaRIII (CD16) triggers effective lysis of lymphoma cells. Br J Haematol. 2004 Apr; 125(2):167-79. PMID:15059139
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Fc gamma receptor III (Catalog #AAA488349) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Fc gamma receptor III [3G8] reacts with Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset and may cross-react with other species as described in the data sheet. AAA Biotech's Fc gamma receptor III can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR), Depletion, Immunoprecipitation (IP), Immunohistochemistry (IHC), Block (BL). 3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies. Researchers should empirically determine the suitability of the Fc gamma receptor III for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fc gamma receptor III, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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