Rabbit SFTPC Polyclonal Antibody | anti-SFTPC antibody

SFTPC Antibody

Cat. Num. AAA9408251

• Gene Names: SFTPC; SP-C; PSP-C; SFTP2; SMDP2; BRICD6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: SFTPC; Polyclonal Antibody; SFTPC Antibody; SP-C; PSP-C; SFTP2; SMDP2; BRICD6; anti-SFTPC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total SFTPC protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 191

• Applicable Applications for anti-SFTPC antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human SFTPC.
• Target Name: SFTPC
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, usingSFTPC antibody at 1:1000 dilution.Secondary antibody: HRPGoat Anti-Rabbit IgG (H+L) at 1:10000dilution.Lysates/proteins: 25ug per lane. Blocking bu?¬€er:3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of para?¬ƒn-embedded human lungusing SFTPC antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of para?¬ƒn-embedded rat lung usingSFTPC antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of para?¬ƒn-embedded human lungusing SFTPC antibody at dilution of 1:200 (40x lens).Immunohistochemistry of para?¬ƒn-embedded rat lung usingSFTPC antibody at dilution of 1:200 (40x lens).Immunohistochemistry of para?¬ƒn-embedded mouse lungusing SFTPC antibody at dilution of 1:200 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of human lung cancer usingSFTPC Polyclonal Antibody at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of mouse lung using SFTPCPolyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Related Product Information for anti-SFTPC antibody

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.

Product Categories/Family for anti-SFTPC antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 288915543
• NCBI GeneID: 6440
• NCBI Accession #: NP_001165828.1
• NCBI GenBank Nucleotide #: NM_001172357.1
• UniProt Accession #: P11686; P11687; Q12793; Q7Z5D0; A6XNE4; B2RE00; E9PGX3
• Molecular Weight: SDS-PAGE MW: 21KD
• NCBI Official Full Name: pulmonary surfactant-associated protein C isoform 2 proprotein
• NCBI Official Synonym Full Names: surfactant protein C
• NCBI Official Symbol: SFTPC
• NCBI Official Synonym Symbols: SP-C; PSP-C; SFTP2; SMDP2; BRICD6
• NCBI Protein Information: pulmonary surfactant-associated protein C
• UniProt Protein Name: Pulmonary surfactant-associated protein C
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPC
• UniProt Synonym Gene Names: SFTP2; SP-C
• UniProt Entry Name: PSPC_HUMAN

NCBI Description

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]

Uniprot Description

SFTPC: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2); also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: multivesicular body; extracellular space

Molecular Function: protein binding; protein homodimerization activity

Biological Process: circadian rhythm; response to cAMP; response to hyperoxia; response to retinoic acid; response to glucocorticoid stimulus; response to glucose stimulus; response to lipopolysaccharide; respiratory gaseous exchange; protein homooligomerization; response to vitamin A

Disease: Pulmonary Fibrosis, Idiopathic; Surfactant Metabolism Dysfunction, Pulmonary, 2