Mouse anti-Human HAUSP Monoclonal Antibody | anti-HAUSP antibody

Anti-HAUSP Mouse mAb

Cat. Num. AAA476623

• Gene Names: Usp7; Hausp
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: HAUSP; Monoclonal Antibody; Anti-HAUSP Mouse mAb; GIG13; anti-HAUSP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5D5B8
• Form/Format: Purified antibody from tissue culture in PBS with 0.05% sodium azide
• Sequence Length: 1103

• Applicable Applications for anti-HAUSP antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Immunogen: Purified recombinant fragment of human PER3 (AA: 723-954) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using HAUSP mouse mAb against MCF-7 (1) cell lysate.)

Related Product Information for anti-HAUSP antibody

Entrez Summary: USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP acitivity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.

NCBI and Uniprot Product Information

• NCBI GI #: 49823179
• NCBI GeneID: 360471
• Molecular Weight: 128
• NCBI Official Full Name: HAUSP
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 7
• NCBI Official Symbol: Usp7
• NCBI Official Synonym Symbols: Hausp
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 7
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 7
• UniProt Gene Name: Usp7
• UniProt Synonym Gene Names: Hausp; rHAUSP

Uniprot Description

USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 10q12

Cellular Component: cytoplasm; cytosol; nuclear body; nucleus; XY body

Molecular Function: cysteine-type endopeptidase activity; p53 binding; protein C-terminus binding; protein homodimerization activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: inhibition of NF-kappaB transcription factor; maintenance of DNA methylation; positive regulation of apoptosis; protein deubiquitination; protein stabilization; regulation of protein stability; regulation of transcription factor activity; transcription-coupled nucleotide-excision repair