Western Blot (WB)
(Western blot detection of PSMB8/LMP7 in C6, Hela, Molt4 and Raji cell lysates using PSMB8/LMP7 mouse mAb (1:1000 diluted).Predicted band size:30KDa.Observed band size:23KDa.)
Mouse anti-Human, Rat PSMB8/LMP7 Monoclonal Antibody | anti-PSMB8/LMP7 antibody
Anti-PSMB8/LMP7 Mouse mAb
Western Blot (WB)
(Western blot detection of PSMB8/LMP7 in C6, Hela, Molt4 and Raji cell lysates using PSMB8/LMP7 mouse mAb (1:1000 diluted).Predicted band size:30KDa.Observed band size:23KDa.)
Western Blot (WB)
(Western blot detection of PSMB8/LMP7 in C6, Hela, Molt4 and Raji cell lysates using PSMB8/LMP7 mouse mAb (1:1000 diluted).Predicted band size:30KDa.Observed band size:23KDa.)
Western Blot (WB)
(Western blot analysis using PSMB8 mouse mAb against Hela (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate.)
Western Blot (WB)
(Western blot analysis using PSMB8 mouse mAb against Hela (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate.)
Immunofluorescence (IF)
(Immunofluorescence analysis of Hela cells using PSMB8 mouse mAb (green). Blue)
Immunofluorescence (IF)
(Immunofluorescence analysis of Hela cells using PSMB8 mouse mAb (green). Blue)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded intima cancer tissues using PSMB8 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded intima cancer tissues using PSMB8 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded colon tissues using PSMB8 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded colon tissues using PSMB8 mouse mAb with DAB staining.)
UniPort Summary Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
NCBI and Uniprot Product Information
NCBI Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]
Uniprot Description
PSMB8: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternatively spliced isoforms have been identified; both isoforms are processed to yield the same mature subunit.
Protein type: EC 3.4.25.1; Protease; Proteasome complex
Chromosomal Location of Human Ortholog: 6p21.32
Cellular Component: cytosol; nucleoplasm; proteasome complex; proteasome core complex
Molecular Function: protein binding
Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; fat cell differentiation; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of endopeptidase activity; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
Disease: Autoinflammation, Lipodystrophy, And Dermatosis Syndrome
Research Articles on PSMB8/LMP7
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Product Notes
The PSMB8/LMP7 psmb8 (Catalog #AAA476203) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMB8/LMP7 Mouse mAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PSMB8/LMP7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PSMB8/LMP7 psmb8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMB8/LMP7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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