Rabbit anti-Human, Mouse FADD Polyclonal Antibody | anti-FADD antibody

FADD Rabbit pAb

Cat. Num. AAA4757787

• Gene Names: FADD; GIG3; MORT1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: FADD; Polyclonal Antibody; FADD Rabbit pAb; Fas (TNFRSF6)-associated via death domain; GIG3; MORT1; anti-FADD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. Gene expression databases
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-FADD antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:2000-1:10,000; IHC: 1:50-1:100
• Immunogen: Fusion protein of human FADD
• Conjugation: Unconjugated
• Molecular Function: Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Gel: 8+12%SDS-PAGE, Lysate: 30 ug, Lane 1-2: Mouse spleen tissue, RAW264.7 cells, Primary antibody: 820622(FADD Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 820622(FADD Antibody) at dilution 1/35. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 820622(FADD Antibody) at dilution 1/35. (Original magnification: ×200))

Related Product Information for anti-FADD antibody

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Product Categories/Family for anti-FADD antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 4505229
• NCBI GeneID: 8772
• NCBI Accession #: NP_003815.1
• NCBI GenBank Nucleotide #: NM_003824.3
• UniProt Accession #: Q13158; Q14866; Q6IBR4
• Molecular Weight: 23,279 Da
• NCBI Official Full Name: protein FADD
• NCBI Official Synonym Full Names: Fas (TNFRSF6)-associated via death domain
• NCBI Official Symbol: FADD
• NCBI Official Synonym Symbols: GIG3; MORT1
• NCBI Protein Information: protein FADD; growth-inhibiting gene 3 protein; mediator of receptor induced toxicity; mediator of receptor-induced toxicity; Fas-associating protein with death domain; Fas-associating death domain-containing protein
• UniProt Protein Name: Protein FADD
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: FADD
• UniProt Synonym Gene Names: MORT1
• UniProt Entry Name: FADD_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]

Uniprot Description

FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: neuron projection; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; protease binding; death receptor binding; protein complex binding; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: viral reproduction; positive regulation of proteolysis; protein heterooligomerization; apoptosis; positive regulation of apoptosis; positive regulation of T cell mediated cytotoxicity; T cell differentiation in the thymus; toll-like receptor 3 signaling pathway; positive regulation of activated T cell proliferation; positive regulation of interleukin-8 production; T cell homeostasis; positive regulation of macrophage differentiation; defense response to virus; toll-like receptor 4 signaling pathway; positive regulation of adaptive immune response; caspase activation; spleen development; positive regulation of I-kappaB kinase/NF-kappaB cascade; thymus development; MyD88-independent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor production; lymph node development; positive regulation of interferon-gamma production; induction of apoptosis via death domain receptors; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter

Disease: Infections, Recurrent, With Encephalopathy, Hepatic Dysfunction, And Cardiovascular Malformations