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Western Blot (WB) (Western blot detection of Vimentin in Jurkat, A549, MCF7 and Hela cell lysates using Vimentin mouse mAb (1:5000 diluted).Predicted band size:57KDa.Observed band size:57KDa.)

Mouse anti-Human Vimentin Monoclonal Antibody | anti-VIM antibody

Anti-Vimentin Mouse mAb

Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human
Applications
Western Blot
Synonyms
Vimentin; Monoclonal Antibody; Anti-Vimentin Mouse mAb; CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; OTTHUMP00000019224; VIM; VIME_HUMAN; anti-VIM antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4F2-H7-A1
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
466
Applicable Applications for anti-VIM antibody
Western Blot (WB)
Immunogen
Purified recombinant fragment of Vimentin expressed in E Coli.
Sequence Similarities
Belongs to the intermediate filament family.
Subunit Structure
Homopolymer assembled from elementary dimers. Interacts with HCV core protein (PubMed:15846844). Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with SLC6A4 (PubMed:19270731). Interacts with STK33 (PubMed:18811945). Interacts with LARP6 (PubMed:21746880). Interacts with RAB8B (By similarity). Interacts with TOR1A; the interaction associates TOR1A with the cytoskeleton (PubMed:16361107, PubMed:18827015). Interacts with TOR1AIP1 (PubMed:16361107). Interacts with BCAS3 (PubMed:17505058). Interacts with DIAPH1 (PubMed:23325789). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity).
Subcellular Location
Cytoplasm

Western Blot (WB)

(Western blot detection of Vimentin in Jurkat, A549, MCF7 and Hela cell lysates using Vimentin mouse mAb (1:5000 diluted).Predicted band size:57KDa.Observed band size:57KDa.)

Western Blot (WB) (Western blot detection of Vimentin in Jurkat, A549, MCF7 and Hela cell lysates using Vimentin mouse mAb (1:5000 diluted).Predicted band size:57KDa.Observed band size:57KDa.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining.)
Related Product Information for anti-VIM antibody
Entrez Summary: This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
UniPort Summary Function: Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57kDa
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Official Synonym Symbols
HEL113; CTRCT30
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
Protein Family
UniProt Gene Name
VIM

NCBI Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Uniprot Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Research Articles on VIM

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Product Notes

The VIM vim (Catalog #AAA475729) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Vimentin Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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