Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-VIM-S56 antibody at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours or treated by Paclitaxel (100nM/ml) for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
Rabbit anti-Human, Rat VIM-S56 Polyclonal Antibody | anti-VIM-S56 antibody
Phospho-VIM-S56 pAb
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-VIM-S56 antibody at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours or treated by Paclitaxel (100nM/ml) for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-VIM-S56 antibody at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours or treated by Paclitaxel (100nM/ml) for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
NCBI and Uniprot Product Information
Observed: 57kDa
NCBI Description
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Uniprot Description
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Research Articles on VIM-S56
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Product Notes
The VIM-S56 vim (Catalog #AAA9135657) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-VIM-S56 pAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VIM-S56 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the VIM-S56 vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ASSPG. It is sometimes possible for the material contained within the vial of "VIM-S56, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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