Rabbit anti-Human B7H4 Polyclonal Antibody | anti-B7H4 antibody

B7H4 Rabbit pAb

Cat. Num. AAA4756048

• Gene Names: VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: B7H4; Polyclonal Antibody; B7H4 Rabbit pAb; V-set domain-containing T-cell activation inhibitor 1; B7X; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; anti-B7H4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-B7H4 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; IHC: 1:20-1:500; ICC/IF: 1:50
• Immunogen: Recombinant protein of human B7H4
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Cell membrane, Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of B7H4 in MDA-MB-468, T47D cell lysates using B7H4 Rabbit pAb(1:1000 diluted).Predicted band size:31kDa.Observed band size:70kDa.)

Related Product Information for anti-B7H4 antibody

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Product Categories/Family for anti-B7H4 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 70997756
• NCBI GeneID: 79679
• UniProt Accession #: Q7Z7D3; Q0GN76; Q45VN0; Q5WPZ3; Q6P097; Q9H6B2
• Molecular Weight: 30,878 Da
• NCBI Official Full Name: B7-H4
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: VTCN1
• NCBI Official Synonym Symbols: B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
• NCBI Protein Information: V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
• UniProt Protein Name: V-set domain-containing T-cell activation inhibitor 1
• UniProt Gene Name: VTCN1
• UniProt Synonym Gene Names: B7H4
• UniProt Entry Name: VTCN1_HUMAN

NCBI Description

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: receptor binding

Biological Process: response to protozoan; immune system process; positive regulation of T cell proliferation; negative regulation of T cell activation