Testing Data
(Detection of human C3 was performed using Mouse anti Human C3 at 0.5 ug/ml followed by Rabbit F(ab')2 anti Mouse IgG-HRP at 1:2000. Lane 2 shows the C3 alpha chain at molecular weight of approximately 115 kDa and the C3c alpha chain fragment 2 at approximately 39 kDa. Lane 5 and 6 shows the mature C3 at molecular weight of approximately 186 kDa.Human plasma was run under reducing conditions at dilutions of 1/20 and 1/200 respectively in lanes 2 and 3, and under non-reducing conditions at dilutions of 1/20 and 1/200 in lanes 5 and 6, respectively. Lane 4 was left blank)
Mouse anti-Human C3 Monoclonal Antibody | anti-C3 antibody
Mouse Anti Human C3
Under reducing conditions the C3 alpha chain at an approximate molecular weight of 115 kDa and the C3c alpha chain fragment 2 at approximately 39 kDa can be detected by Western blotting. Under non reducing conditions the mature C3 band can be detected at a molecular weight of approximately 186 kDa.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Detection of human C3 was performed using Mouse anti Human C3 at 0.5 ug/ml followed by Rabbit F(ab')2 anti Mouse IgG-HRP at 1:2000. Lane 2 shows the C3 alpha chain at molecular weight of approximately 115 kDa and the C3c alpha chain fragment 2 at approximately 39 kDa. Lane 5 and 6 shows the mature C3 at molecular weight of approximately 186 kDa.Human plasma was run under reducing conditions at dilutions of 1/20 and 1/200 respectively in lanes 2 and 3, and under non-reducing conditions at dilutions of 1/20 and 1/200 in lanes 5 and 6, respectively. Lane 4 was left blank)
Testing Data
(Detection of human C3 was performed using Mouse anti Human C3 at 0.5 ug/ml followed by Rabbit F(ab')2 anti Mouse IgG-HRP at 1:2000. Lane 2 shows the C3 alpha chain at molecular weight of approximately 115 kDa and the C3c alpha chain fragment 2 at approximately 39 kDa. Lane 5 and 6 shows the mature C3 at molecular weight of approximately 186 kDa.Human plasma was run under reducing conditions at dilutions of 1/20 and 1/200 respectively in lanes 2 and 3, and under non-reducing conditions at dilutions of 1/20 and 1/200 in lanes 5 and 6, respectively. Lane 4 was left blank)
The central position of C3 in the cascade has led to the emergence of immune invasion strategies by many human pathogens, including Staphylococcus aureus which expresses 2 proteins that interfere with the activation of C3 (Garcia et al. 2012). C3 Deficiencies in humans also show an increased susceptibility to bacterial infections in early childhood the development of autoimmune disease and kidney diseases (Ricklin et al. 2016).
Conversely, complement over-activation can result in various disease pathologies, including induction of inflammatory pathways causing tissue damage. With complements' role in various diseases, pharmaceutical and biotech companies have targeted the cascade for potential therapeutic targets in a range of immune and inflammatory diseases as well as in transplantation medicine (Ricklin et al. 2012).
C3 is activated via its' cleavage into fragments which have varying functions within the complement cascade.
NCBI and Uniprot Product Information
NCBI Description
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. [provided by RefSeq, Nov 2015]
Uniprot Description
C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
Protein type: Secreted, signal peptide; Secreted; Inhibitor
Chromosomal Location of Human Ortholog: 19p13.3-p13.2
Cellular Component: extracellular space; plasma membrane; extracellular region
Molecular Function: protein binding; endopeptidase inhibitor activity; C5L2 anaphylatoxin chemotactic receptor binding; receptor binding
Biological Process: regulation of immune response; complement activation, alternative pathway; signal transduction; fatty acid metabolic process; complement activation; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of type IIa hypersensitivity; regulation of complement activation; innate immune response; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response; complement activation, classical pathway
Disease: Complement Component 3 Deficiency, Autosomal Recessive; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 5; Macular Degeneration, Age-related, 9
Research Articles on C3
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Product Notes
The C3 c3 (Catalog #AAA225710) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human C3 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Under reducing conditions the C3 alpha chain at an approximate molecular weight of 115 kDa and the C3c alpha chain fragment 2 at approximately 39 kDa can be detected by Western blotting. Under non reducing conditions the mature C3 band can be detected at a molecular weight of approximately 186 kDa. Researchers should empirically determine the suitability of the C3 c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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