Rabbit PRMT7 Monoclonal Antibody | anti-PRMT7 antibody

PRMT7 Rabbit mAb

Cat. Num. AAA4752976

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: PRMT7; Monoclonal Antibody; PRMT7 Rabbit mAb; Protein arginine N-methyltransferase 7; SBIDDS; anti-PRMT7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R06-4F6
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-PRMT7 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:5000
• Immunogen: A synthetic peptide of human PRMT7
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cytoplasm, Nucleus
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of PRMT7 in K562,3T3,Hela cell lysates using PRMT7 Rabbit mAb(1:1000 diluted). Predicted band size:79kDa. Observed band size:79kDa.)

Related Product Information for anti-PRMT7 antibody

This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017]
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 9506615
• NCBI GeneID: 54496
• NCBI Accession #: NP_061896.1
• NCBI GenBank Nucleotide #: NM_019023.2
• UniProt Accession #: Q9NVM4; Q96PV5; Q9H9L0; B3KPR0; B3KUG9; B4E379
• Molecular Weight: 63,806 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 7 isoform 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 7
• NCBI Official Symbol: PRMT7
• NCBI Protein Information: protein arginine N-methyltransferase 7; histone-arginine N-methyltransferase PRMT7; myelin basic protein-arginine N-methyltransferase
• UniProt Protein Name: Protein arginine N-methyltransferase 7
• UniProt Gene Name: PRMT7
• UniProt Synonym Gene Names: KIAA1933
• UniProt Entry Name: ANM7_HUMAN

NCBI Description

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM, Mar 2008]

Uniprot Description

PRMT7: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.126; EC 2.1.1.125; Methyltransferase, protein arginine; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: ribonucleoprotein binding; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; [myelin basic protein]-arginine N-methyltransferase activity; histone binding; protein-arginine omega-N symmetric methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity; histone-arginine N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; spliceosomal snRNP biogenesis; peptidyl-arginine methylation; genetic imprinting; regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA methylation during gametogenesis; regulation of protein binding; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; cell differentiation