Rabbit anti-Human, Mouse PRMT7 Polyclonal Antibody | anti-PRMT7 antibody

PRMT7 Antibody

Cat. Num. AAA7051436

• Gene Names: PRMT7; SBIDDS
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: PRMT7; Polyclonal Antibody; PRMT7 Antibody; Protein arginine N-methyltransferase 7; Histone-arginine N-methyltransferase PRMT7; [Myelin basic protein]-arginine N-methyltransferase PRMT7; KIAA1933; anti-PRMT7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 642

• Applicable Applications for anti-PRMT7 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Protein arginine N-methyltransferase 7 protein (285-390AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-48657 / sc-48658 / sc-48660 / sc-98882
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse spleen tissue,Mouse lung tissue,Mouse kidney tissue,Mouse brain tissueAll lanes: PRMT7 antibody at 6.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 79,63,74,64 kDaObserved band size: 79 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7051436 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7051436 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-PRMT7 antibody

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 296317343
• NCBI GeneID: 54496
• NCBI Accession #: NP_001171753.1
• NCBI GenBank Nucleotide #: NM_001184824.1
• UniProt Accession #: Q9NVM4; Q96PV5; Q9H9L0; B3KPR0; B3KUG9; B4E379
• Molecular Weight: 63,806 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 7 isoform 2
• NCBI Official Synonym Full Names: protein arginine methyltransferase 7
• NCBI Official Symbol: PRMT7
• NCBI Official Synonym Symbols: SBIDDS
• NCBI Protein Information: protein arginine N-methyltransferase 7
• UniProt Protein Name: Protein arginine N-methyltransferase 7
• UniProt Gene Name: PRMT7
• UniProt Synonym Gene Names: KIAA1933
• UniProt Entry Name: ANM7_HUMAN

NCBI Description

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM, Mar 2008]

Uniprot Description

PRMT7: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.125; EC 2.1.1.126; Methyltransferase; Methyltransferase, protein arginine; RNA processing

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cytosol; nucleolus; nucleoplasm; nucleus

Molecular Function: [myelin basic protein]-arginine N-methyltransferase activity; histone binding; histone-arginine N-methyltransferase activity; protein-arginine N-methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein-arginine omega-N symmetric methyltransferase activity; ribonucleoprotein binding; S-adenosylmethionine-dependent methyltransferase activity

Biological Process: DNA methylation during gametogenesis; genetic imprinting; histone methylation; peptidyl-arginine methylation; regulation of protein binding; regulation of transcription, DNA-dependent; spliceosomal snRNP biogenesis

Disease: Short Stature, Brachydactyly, Intellectual Developmental Disability, And Seizures