CCL26/Eotaxin-3 Recombinant Protein | CCL26 recombinant protein

CCL26/Eotaxin-3 Protein, Human, Recombinant

Cat. Num. AAA8120368

• Gene Names: CCL26; IMAC; TSC-1; MIP-4a; SCYA26; MIP-4alpha
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CCL26/Eotaxin-3; CCL26/Eotaxin-3 Protein; Human; Recombinant; Human CCL26/Eotaxin-3 Protein; IMAC Protein; MIP-4a Protein; MIP-4alpha Protein; SCYA26 Protein; TSC-1 Protein; chemokine (C-C motif) ligand 26; CCL26 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 300mM NaCl, pH8.0, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu94

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Thr 24
• Tag: Native
• Protein Construction: A DNA sequence encoding the human CCL26 (NP_006063.1)(Met1-Leu94) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL26 recombinant protein

Background: The eotaxin subfamily of CC chemokines consists of eotaxin-1/CCL11, eotaxin-2/CCL24 and eotaxin-3/CCL26. All eotaxins induce the trafficking of eosinophils to the sites of inflammation via CC chemokine receptor 3 (CCR3), which is also expressed by several different cell types, including basophils, dendritic cells, smooth muscle cells, epithelial cells and fibroblasts. The sequence similarity between the three eotaxins is limited (<4%), but their functional properties are very similar. Eotaxin-1 and -2 are expressed by both haematopoietic and non-haematopoietic cells, but eotaxin-3 expression has been reported to be limited to non-haematopoietic cells. Interleukin (IL)-4 is the main inducer for eotaxin-3 expression, whereas eotaxin-1 is up-regulated by IL-4 and the proinflammatory cytokine tumour necrosis factor (TNF)-alpha. Eotaxin-3 is expressed in vascular endothelial cells and human dermal fibroblasts after IL-4 and IL-13 stimulation, and this is dependent upon the IL-4-/IL-13-specific transcription factor, signal transducers and activator of transcription (STAT)-6. Eotaxin-3 is expressed on the surface of IL-4-stimulated endothelial cells and promotes eosinophil transmigration.

References

Takahashi K, Imaeda H, Fujimoto T, et al. Regulation of eotaxin-3/CC chemokine ligand 26 expression by T helper type 2 cytokines in human colonic myofibroblasts. Clinical and Experimental Immunology. 2013;173(2):323-331.

NCBI and Uniprot Product Information

• NCBI GI #: 5174671
• NCBI GeneID: 10344
• NCBI Accession #: NP_006063.1
• NCBI GenBank Nucleotide #: NM_006072.4
• UniProt Accession #: Q9Y258; Q52LV8; A0N0Q5
• Molecular Weight: 10,648 Da
• NCBI Official Full Name: C-C motif chemokine 26
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 26
• NCBI Official Symbol: CCL26
• NCBI Official Synonym Symbols: IMAC; TSC-1; MIP-4a; SCYA26; MIP-4alpha
• NCBI Protein Information: C-C motif chemokine 26; CC chemokine IMAC; MIP-4-alpha; chemokine N1; eotaxin-3; macrophage inflammatory protein 4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; small-inducible cytokine A26; thymic stroma
• UniProt Protein Name: C-C motif chemokine 26
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL26
• UniProt Synonym Gene Names: SCYA26; MIP-4-alpha; TSC-1
• UniProt Entry Name: CCL26_HUMAN

NCBI Description

This gene is one of two Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 7. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for normal peripheral blood eosinophils and basophils. The product of this gene is one of three related chemokines that specifically activate chemokine receptor CCR3. This chemokine may contribute to the eosinophil accumulation in atopic diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL26: Chemotactic for eosinophils and basophils. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cell-cell signaling; positive regulation of actin filament polymerization; immune response; positive regulation of endothelial cell proliferation; chemotaxis; inflammatory response; signal transduction; positive regulation of cell migration