Western Blot (WB)
(Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).)
IgG secondary antibody
Anti-Rat IgG Light Chain:HRP Secondary Antibody
Reactivity
Rat
Applications
Western Blot, ELISA, Immunocytochemistry, Immunohistochemistry
Synonyms
IgG; Polyclonal Secondary Antibody; Anti-Rat IgG Light Chain:HRP Secondary Antibody; Anti-Rat IgG Light Chain:HRP; ZZ Rt LC; IgG secondary antibody
Host
Goat
Reactivity
Rat
Clonality
Polyclonal
Specificity
This antibody has been pre-adsorbed with various immunoglobulins from non-rat species before affinity-purification using rat IgG light chain coupled to agarose beads. Purified goat polyclonal antibody was conjugated to horseradish peroxidase (HRP). This secondary reagent can be used to detect rat immunoglobulin light chains in various antibody applications that utilize HRP readouts, such as western blot, ELISA, immunocytochemistry, and immunohistochemistry.
Form/Format
Goat polyclonal antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, and 1mg/ml BSA. Note: Use of sodium azide as preservative substantially inhibits the enzyme activity of HRP.
Applicable Applications for IgG secondary antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:5000
ICC: 1:1000
IHC: 1:1000
WB: 1:5000
ICC: 1:1000
IHC: 1:1000
WB: 1:5000
Immunogen
The HRP-conjugated goat polyclonal secondary reagent reacts with the light chains of rat IgG, and the light chains of other rat immunoglobulins. The secondary reagent has minimal cross-reactivity with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).)
Western Blot (WB)
(Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).)
Related Product Information for IgG secondary antibody
An array of chromogenic, fluorogenic and chemiluminescent substrates are available for use with HRP conjugated secondary antibodies HRP is a 40kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. HRP functions optimally at a near-neutral pHand can be inhibited by cyanides, sulfides and azides. Antibody-HRP conjugates are superior to antibody-AP conjugates with respect to the specific activities of both the enzyme and antibody. In addition, its high turnover rate, good stability, low cost and wide availability of substrates makes HRP the enzyme of choice for most applications. HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry.
References
Madamanchi, N.R. & Runge, M.S. (2001) Methods Mol Med. 51:245.
Mattson, D.L. & Bellehumeur, T.G. (1996). Anal. Biochem. 240:306.
Mattson, D.L. & Bellehumeur, T.G. (1996). Anal. Biochem. 240:306.
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Product Notes
The IgG (Catalog #AAA474557) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat IgG Light Chain:HRP Secondary Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC). ELISA: 1:5000 ICC: 1:1000 IHC: 1:1000 WB: 1:5000. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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