E-Cadherin Phospho-Regulation Antibody Sampler Kit
E-Cadherin Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
E-Cadherin Phospho-Regulation; Cadherin EPK; E-Cadherin Phospho-Regulation antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for E-Cadherin Phospho-Regulation antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for E-Cadherin Phospho-Regulation antibody sampler kit
Kit Summary: The E-cadherin phospho-regulation antibody sampler kit can be used to examine phosphorylation of E-cadherin at Tyr-835. The kit includes monoclonal and polyclonal antibodies to monitor the total level of expression for E-Cadherin andsecondary reagents for detection of these antibodies.
Background: Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation.Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediatedadhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the proteinp120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulatethe cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated byc-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 (Tyr-835 in E-cadherin) can disruptcadherin binding to beta-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation ofthese sites may be critical for cadherin function.
Background: Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation.Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediatedadhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the proteinp120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulatethe cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated byc-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 (Tyr-835 in E-cadherin) can disruptcadherin binding to beta-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation ofthese sites may be critical for cadherin function.
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Product Notes
The Human, Rat, Mouse E-Cadherin Phospho-Regulation (Catalog #AAA474207) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474207 Antibody Sampler Kit recognizes Human, Rat, Mouse E-Cadherin Phospho-Regulation. AAA Biotech's E-Cadherin Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the E-Cadherin Phospho-Regulation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E-Cadherin Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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