Western Blot (WB)
(Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Annexin A6 antibody at 1:250 (lane 1) or 1:1000 (lane 2).)
Mouse Annexin A6 Monoclonal Antibody | anti-A6 antibody
Annexin A6 Antibody
Gene Names
Anxa6; Anx6; CPB-II
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
Synonyms
Annexin A6; Monoclonal Antibody; Annexin A6 Antibody; anti-A6 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M330
Specificity
This antibody detects a 67kDa* protein corresponding to the molecular mass of Annexin A6 on SDS-PAGE immunoblots of human Jurkat cells.
Form/Format
Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-A6 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
IP: 1:50
WB: 1:1000
ICC: 1:100
IP: 1:50
WB: 1:1000
ICC: 1:100
Immunogen
Clone (M330) was generated from a full length recombinant rat Annexin A6 protein.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Annexin A6 antibody at 1:250 (lane 1) or 1:1000 (lane 2).)
Western Blot (WB)
(Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Annexin A6 antibody at 1:250 (lane 1) or 1:1000 (lane 2).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of Annexin A6 in human MDA-MB-231 carcinoma cells. The cells were fixed in aldehydes and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A6 (AM3301). The antibody was detected using goat anti-mouse DyLight 594.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of Annexin A6 in human MDA-MB-231 carcinoma cells. The cells were fixed in aldehydes and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A6 (AM3301). The antibody was detected using goat anti-mouse DyLight 594.)
Related Product Information for anti-A6 antibody
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
References
Grewal, T. & Enrich, C. (2006) Bioessays 28(12):1211.
Feinberg, J.M. et al. (1991) J Histochem. & Cytochem. 39:955.
Zokas, L. & Glenney, J.R. (1987) J Cell Biol. 105:2111.
Feinberg, J.M. et al. (1991) J Histochem. & Cytochem. 39:955.
Zokas, L. & Glenney, J.R. (1987) J Cell Biol. 105:2111.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75,754 Da
NCBI Official Full Name
annexin A6
NCBI Official Synonym Full Names
annexin A6
NCBI Official Symbol
Anxa6
NCBI Official Synonym Symbols
Anx6; CPB-II
NCBI Protein Information
annexin A6; CBP 65/67; annexin-6; annexin VI; lipocortin VI; calphobindin-II; chromobindin-20; 67 kDa calelectrin; calcium-binding protein 65/67; calcium-binding protein CATA 65/67
UniProt Protein Name
Annexin A6
Protein Family
UniProt Gene Name
Anxa6
UniProt Synonym Gene Names
Anx6; CBP 65/67
UniProt Entry Name
ANXA6_RAT
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Product Notes
The A6 anxa6 (Catalog #AAA474173) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Annexin A6 Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC). ELISA: 1:2000 IP: 1:50 WB: 1:1000 ICC: 1:100. Researchers should empirically determine the suitability of the A6 anxa6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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