Human Annexin A6 ELISA Kit | ANXA6 elisa kit

Human Annexin A6 ELISA Kit

Cat. Num. AAA760950

• Gene Names: Anxa6; Anx6; CPB-II
• Reactivity: Human
• Synonyms: Annexin A6; Human Annexin A6 ELISA Kit; ANXA6/Annexin A6/Lipocortin VI/Chromobindin-20/Calphobindin-II/CPB-II/Annexin-6/p70/p68/Protein III/67 kDa calelectrin/Annexin VI/CBP68/Calelectrin/ANX6; ANXA6 elisa kit

• Reactivity: Human
• Sequence Length: 673

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ANXA6 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ANXA6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ANXA6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ANXA6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ANXA6 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 130502086
• NCBI GeneID: 79125
• NCBI Accession #: NP_077070.2
• NCBI GenBank Nucleotide #: NM_024156.2
• Molecular Weight: 75,754 Da
• NCBI Official Full Name: annexin A6
• NCBI Official Synonym Full Names: annexin A6
• NCBI Official Symbol: Anxa6
• NCBI Official Synonym Symbols: Anx6; CPB-II
• NCBI Protein Information: annexin A6
• UniProt Protein Name: Annexin A6
• Protein Family: Annexin
• UniProt Gene Name: Anxa6
• UniProt Synonym Gene Names: Anx6; CBP 65/67
• UniProt Entry Name: ANXA6_RAT

NCBI Description

Ca(2+)-sensitive phospholipid-binding protein that binds CRHSP-28 [RGD, Feb 2006]

Uniprot Description

ANXA6: a calcium/phospholipid-binding protein that may associate with CD21. May regulate the release of Ca(2+) from intracellular stores. Phosphorylated in response to growth factor stimulation. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding; Motility/polarity/chemotaxis

Cellular Component: apical plasma membrane; cytoplasm; focal adhesion; late endosome membrane; lysosomal membrane; melanosome; membrane; mitochondrion; nucleus; perinuclear region of cytoplasm; protein complex; sarcolemma

Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; cholesterol binding; GTP binding; ligand-gated ion channel activity; lipid binding; protein binding; protein complex binding; protein homodimerization activity

Biological Process: calcium ion transport; mitochondrial calcium ion homeostasis; protein homooligomerization; regulation of muscle contraction; secretion