Western Blot (WB)
(Western blot of human A431 cells (lane 1), MDA-MB-231 cells (lane 2), A549 cells (lane 3), LNCaP cells (lane 4), full-length recombinant human annexin A1 (lane 5), and recombinant human annexin A2 (lane 6). The blot was probed with mouse monoclonal anti-Annexin A1 antibody (AM0181) at 1:1000 (lanes 1-6).)
Mouse Annexin A1 Monoclonal Antibody | anti-A1 antibody
Annexin A1 M018 Antibody
Gene Names
ANXA1; ANXA1
Reactivity
Human, Rat, Mouse, Bovine
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry
Synonyms
Annexin A1; Monoclonal Antibody; Annexin A1 M018 Antibody; Annexin A1 M021; anti-A1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Bovine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M018
Specificity
Clone M018 detects a 38kDa* protein corresponding to the molecular mass of Annexin A1 on SDS-PAGE immunoblots of human A431, A549, and HeLa. The antibody also detects full-length recombinant human Annexin A1, but not Annexin A2 or Annexin A5 recombinant proteins. The antibody works for western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation.
Form/Format
Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-A1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
ICC: 1:100
IHC: 1:100
IP: 1:50
WB: 1:1000
IHC: 1:100
IP: 1:50
WB: 1:1000
Immunogen
Clone M018 was generated from full-length bovine recombinant Annexin A1.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of human A431 cells (lane 1), MDA-MB-231 cells (lane 2), A549 cells (lane 3), LNCaP cells (lane 4), full-length recombinant human annexin A1 (lane 5), and recombinant human annexin A2 (lane 6). The blot was probed with mouse monoclonal anti-Annexin A1 antibody (AM0181) at 1:1000 (lanes 1-6).)
Western Blot (WB)
(Western blot of human A431 cells (lane 1), MDA-MB-231 cells (lane 2), A549 cells (lane 3), LNCaP cells (lane 4), full-length recombinant human annexin A1 (lane 5), and recombinant human annexin A2 (lane 6). The blot was probed with mouse monoclonal anti-Annexin A1 antibody (AM0181) at 1:1000 (lanes 1-6).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of Annexin A1 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-Annexin A1 (clone M018). The antibody was detected using goat anti-mouse Ig DyLight 594.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of Annexin A1 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-Annexin A1 (clone M018). The antibody was detected using goat anti-mouse Ig DyLight 594.)
Related Product Information for anti-A1 antibody
The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
References
Boye TL & Nylandsted J (2016) Biol Chem. 397(10):961.
Hoque M et al. (2014) Cell Signal. 26(6):1213.
Feinberg, J.M. et al. (1991) J Histochem & Cytochem. 39:955.
Hoque M et al. (2014) Cell Signal. 26(6):1213.
Feinberg, J.M. et al. (1991) J Histochem & Cytochem. 39:955.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,952 Da
NCBI Official Full Name
annexin A1
NCBI Official Symbol
ANXA1
NCBI Official Synonym Symbols
ANXA1
NCBI Protein Information
annexin A1; p35; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; phospholipase A2 inhibitory protein
UniProt Protein Name
Annexin A1
Protein Family
UniProt Gene Name
ANXA1
UniProt Synonym Gene Names
ANX1
UniProt Entry Name
ANXA1_BOVIN
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Product Notes
The A1 anxa1 (Catalog #AAA474164) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Annexin A1 M018 Antibody reacts with Human, Rat, Mouse, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC). ICC: 1:100 IHC: 1:100 IP: 1:50 WB: 1:1000. Researchers should empirically determine the suitability of the A1 anxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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