Mouse anti-Human BCL-2 Monoclonal Antibody | anti-BCL-2 antibody
MOUSE anti BCL-2alpha (CLONE SPM117)
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Synthetic peptide corresponding to aa 41-54 of human Bcl-2alpha.
Synonyms
BCL-2; Monoclonal Antibody; MOUSE anti BCL-2alpha (CLONE SPM117); Mouse Anti-BCL-2á; anti-BCL-2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
SPM117
Specificity
Expression of Bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. This antibody recognizes human BCL-2alpha, the other species are not tested.
Purity/Purification
Synthetic peptide corresponding to aa 41-54 of human Bcl-2alpha.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-BCL-2 antibody
Immunohistochemistry - Paraffin, Western Blot, Immunoprecipitation
Application Notes
Western Blot: 0.1-1 ug/ml
Immunoprecipitation: 2ug/ml
Immunohistochemistry: 1:50, 2-5 ug/ml
Immunoprecipitation: 2ug/ml
Immunohistochemistry: 1:50, 2-5 ug/ml
Antigen Preparation
A synthetic peptide corresponding to Actin Protein, smooth muscle specific.
Positive Control
Raji cells. Tonsil
Cellular Location
Cytoplasm and cell membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. Upon initial thawing, apportion into working aliquots and store at -70 degree C for up to six months. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
NCBI and Uniprot Product Information
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Product Notes
The BCL-2 (Catalog #AAA462227) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE anti BCL-2alpha (CLONE SPM117) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCL-2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry - Paraffin, Western Blot, Immunoprecipitation. Western Blot: 0.1-1 ug/ml Immunoprecipitation: 2ug/ml Immunohistochemistry: 1:50, 2-5 ug/ml. Researchers should empirically determine the suitability of the BCL-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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