Human F box only protein 34 (FBXO34) ELISA Kit | FBXO34 elisa kit

Human F box only protein 34 (FBXO34) ELISA Kit

Cat. Num. AAA7218562

• Gene Names: FBXO34; Fbx34; CGI-301
• Reactivity: Human
• Synonyms: F box only protein 34 (FBXO34); Human F box only protein 34 (FBXO34) ELISA Kit; FBXO34 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This FBXO34 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FBXO34. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBXO34 elisa kit

Principle of the assay: FBXO34 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FBXO34 antibody and an FBXO34-HRP conjugate. The assay sample and buffer are incubated together with FBXO34-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBXO34 concentration since FBXO34 from samples and FBXO34-HRP conjugate compete for the anti-FBXO34 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBXO34 from the sample, fewer sites are left to bind FBXO34-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBXO34 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FBXO34 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 195222730
• NCBI GeneID: 55030
• NCBI Accession #: NP_689417.1
• NCBI GenBank Nucleotide #: NM_152231.1
• Molecular Weight: 78,711 Da
• NCBI Official Full Name: F-box only protein 34
• NCBI Official Synonym Full Names: F-box protein 34
• NCBI Official Symbol: FBXO34
• NCBI Official Synonym Symbols: Fbx34; CGI-301
• NCBI Protein Information: F-box only protein 34; protein CGI-301
• UniProt Protein Name: F-box only protein 34
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO34
• UniProt Synonym Gene Names: FBX34
• UniProt Entry Name: FBX34_HUMAN

NCBI Description

Members of the F-box protein family, such as FBXO34, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

Uniprot Description

FBXO34: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: nucleoplasm; cytoplasm