Rabbit anti-Human DBF4B Polyclonal Antibody | anti-DBF4B antibody

Rabbit anti DBF4B Polyclonal Antibody

Cat. Num. AAA460402

• Gene Names: DBF4B; DRF1; ASKL1; CHIFB; ZDBF1B
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: DBF4B; Polyclonal Antibody; Rabbit anti DBF4B Polyclonal Antibody; DBF4 homolog B; anti-DBF4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~77 kDa of DBF4 protein. It cross reacts to human, mouse and rat. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 431

• Applicable Applications for anti-DBF4B antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to the N-terminus of DBF4B protein. This sequence is identical to human, rat and mouse.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-DBF4B antibody

The human DBF4/ASK protein contains a putative site for phosphorylation by CDK, PEST protease cleavage sites, nuclear localization signals and a short-looped zinc finger-like domain. DBF4 interacts physically with CDC7 and MCM2 as a cyclin-like regulatory subunit of mammalian CDC7, thus it plays a key role in the regulation of DNA replication during the cell cycle in all eukaryotes. The DBF4/ASK is also identified as a novel determinant for melanoma development. The up-regulation of ASK/DBF4 is a novel molecular determinant with prognostic relevance that confers a proliferative advantage in melanoma.

References

Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M. and Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Mol. Gen. Genet. 262 (2), 220-229 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 16740566
• NCBI GeneID: 80174
• Molecular Weight: 18,392 Da
• NCBI Official Full Name: DBF4B protein
• NCBI Official Synonym Full Names: DBF4 zinc finger B
• NCBI Official Symbol: DBF4B
• NCBI Official Synonym Symbols: DRF1; ASKL1; CHIFB; ZDBF1B
• NCBI Protein Information: protein DBF4 homolog B
• UniProt Protein Name: Protein DBF4 homolog B
• Protein Family: Protein
• UniProt Gene Name: DBF4B
• UniProt Synonym Gene Names: ASKL1; DRF1; ASK-like protein 1

NCBI Description

This gene encodes a regulator of the cell division cycle 7 homolog (S. cerevisiae) protein, a serine-threonine kinase which links cell cycle regulation to genome duplication. This protein localizes to the nucleus and, in complex with the cell division cycle 7 homolog (S. cerevisiae) protein, may facilitate M phase progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Uniprot Description

DBF4B: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q21.31|17q21

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nuclear chromatin; nucleoplasm; nucleus

Molecular Function: protein binding; protein kinase activator activity; protein kinase binding

Biological Process: positive regulation of cell proliferation