Mouse Coagulation Factor VII (F7) RTU ELISA Kit | F7 rtu elisa kit

Mouse Coagulation Factor VII (F7) ELISA Kit

Cat. Num. AAA459151

• Gene Names: F7; Cf7; FVII; AI132620
• Reactivity: Mouse
• Synonyms: Coagulation Factor VII (F7); Mouse Coagulation Factor VII (F7) ELISA Kit; FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa; F7 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 446

• Assay Type: Sandwich
• Samples: Plasma.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 37pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F7 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to F7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 225543550
• NCBI GeneID: 14068
• NCBI Accession #: NP_034302.2
• NCBI GenBank Nucleotide #: NM_010172.4
• UniProt Accession #: P70375; Q61109
• Molecular Weight: 50,276 Da
• NCBI Official Full Name: coagulation factor VII preproprotein
• NCBI Official Synonym Full Names: coagulation factor VII
• NCBI Official Symbol: F7
• NCBI Official Synonym Symbols: Cf7; FVII; AI132620
• NCBI Protein Information: coagulation factor VII
• UniProt Protein Name: Coagulation factor VII
• Protein Family: Coagulation factor
• UniProt Gene Name: F7
• UniProt Synonym Gene Names: Cf7

NCBI Description

This gene encodes a vitamin K-dependent serine protease that plays a critical role in the extrinsic pathway of blood coagulation. Upon contact with tissue factor III (TF III), the encoded protein forms an activated complex termed TF-FVIIa that initiates the coagulation cascade involving other coagulation factors, ultimately resulting in a fibrin clot. Complete lack of the encoded protein in mice results in in perinatal lethality due to bleeding from normal blood vessels. [provided by RefSeq, Apr 2015]

Uniprot Description

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium ().