Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) RTU ELISA Kit | CX3CL1 rtu elisa kit

Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

Cat. Num. AAA458695

• Gene Names: Cx3cl1; CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e
• Reactivity: Mouse
• Synonyms: Chemokine C-X3-C-Motif Ligand 1 (CX3CL1); Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit; NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1; CX3CL1 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 395

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.060ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CX3CL1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CX3CL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CX3CL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CX3CL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CX3CL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31982017
• NCBI GeneID: 20312
• NCBI Accession #: NP_033168.2
• NCBI GenBank Nucleotide #: NM_009142.3
• UniProt Accession #: O35188; O35933; Q91V44
• Molecular Weight: 42,099 Da
• NCBI Official Full Name: fractalkine
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: Cx3cl1
• NCBI Official Synonym Symbols: CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e
• NCBI Protein Information: fractalkine
• UniProt Protein Name: Fractalkine
• Protein Family: Fractalkine
• UniProt Gene Name: Cx3cl1
• UniProt Synonym Gene Names: Cx3c; Fkn; Scyd1

Uniprot Description

Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (). The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9177350, PubMed:10382755).