Human Acylglycerol Kinase (AGK) RTU ELISA Kit | AGK rtu elisa kit

Human Acylglycerol Kinase (AGK) ELISA Kit

Cat. Num. AAA457478

• Gene Names: AGK; MULK; CATC5; CTRCT38; MTDPS10
• Reactivity: Human
• Synonyms: Acylglycerol Kinase (AGK); Human Acylglycerol Kinase (AGK) ELISA Kit; MULK; Multiple Substrate Lipid Kinase; AGK rtu elisa kit

• Reactivity: Human
• Sequence Length: 422

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.081ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGK rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AGK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AGK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AGK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8922701
• NCBI GeneID: 55750
• NCBI Accession #: NP_060708.1
• NCBI GenBank Nucleotide #: NM_018238.3
• UniProt Accession #: Q53H12; Q75KN1; Q96GC3; Q9NP48
• Molecular Weight: 7,634 Da
• NCBI Official Full Name: acylglycerol kinase, mitochondrial
• NCBI Official Synonym Full Names: acylglycerol kinase
• NCBI Official Symbol: AGK
• NCBI Official Synonym Symbols: MULK; CATC5; CTRCT38; MTDPS10
• NCBI Protein Information: acylglycerol kinase, mitochondrial
• UniProt Protein Name: Acylglycerol kinase, mitochondrial
• Protein Family: Acylglycerol kinase
• UniProt Gene Name: AGK
• UniProt Synonym Gene Names: Multi-substrate lipid kinase

NCBI Description

The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012]

Uniprot Description

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15939762). Does not phosphorylate sphingosine (PubMed:15939762). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (PubMed:28712724, PubMed:28712726). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (PubMed:28712724, PubMed:28712726). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochtondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (PubMed:28712724). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (PubMed:15939762).