Typical Testing Data/Standard Curve (for reference only)
Mouse Drebrin 1 (DBN1) ELISA Kit | DBN1 elisa kit
Mouse Drebrin 1 (DBN1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DBN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DBN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DBN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DBN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
DBN1: a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. Expressed in brain neurons, heart, placenta, skeletal muscle, kidney and pancreas. Two alternatively spliced isoforms have been described.
Protein type: Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 13 B1|13 30.06 cM
Cellular Component: actin cytoskeleton; actin filament; asymmetric synapse; axonal growth cone; axonal spine; cell junction; cell projection; cell soma; cytoplasm; dendritic filopodium; dendritic shaft; dendritic spine; excitatory synapse; filopodium; gap junction; growth cone; intercellular junction; intracellular; lamellipodium; membrane; neuron spine; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; postsynaptic membrane
Molecular Function: actin binding; actin filament binding; cadherin binding; cytoskeletal protein binding; profilin binding; protein binding
Biological Process: actin filament organization; cell communication by chemical coupling; cell communication by electrical coupling; cell differentiation; cellular response to transforming growth factor beta stimulus; cellular response to tumor necrosis factor; generation of neurons; maintenance of cellular protein localization; modulation of inhibitory postsynaptic potential; multicellular organism development; negative regulation of actin filament bundle formation; negative regulation of actin-dependent ATPase activity; negative regulation of locomotion involved in locomotory behavior; negative regulation of muscle filament sliding; nervous system development; positive regulation of axon extension; positive regulation of cell-substrate adhesion; positive regulation of excitatory postsynaptic potential; positive regulation of neuron projection development; positive regulation of substrate adhesion-dependent cell spreading; prepulse inhibition; regulation of actin filament polymerization; regulation of filopodium formation
Research Articles on DBN1
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Product Notes
The Mouse DBN1 dbn1 (Catalog #AAA456850) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456850 ELISA Kit recognizes Mouse DBN1. It is sometimes possible for the material contained within the vial of "Drebrin 1 (DBN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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