Typical Testing Data/Standard Curve (for reference only)
Human ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit | ATP1b1 elisa kit
Human ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP1b1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP1b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP1b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP1b1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]
Uniprot Description
ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Transporter
Chromosomal Location of Human Ortholog: 1q24.2
Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; intracellular; membrane; myelin sheath; plasma membrane; sarcolemma; sodium:potassium-exchanging ATPase complex
Molecular Function: ATP binding; ATPase activator activity; ATPase activity; ATPase binding; drug binding; potassium ion binding; protein binding; protein C-terminus binding; protein kinase binding; sodium ion binding; sodium:potassium-exchanging ATPase activity
Biological Process: ATP metabolic process; cardiac muscle contraction; cell adhesion; cellular calcium ion homeostasis; cellular potassium ion homeostasis; cellular sodium ion homeostasis; establishment or maintenance of transmembrane electrochemical gradient; leukocyte migration; positive regulation of ATPase activity; potassium ion import; protein stabilization; regulation of gene expression; response to hypoxia
Disease: Hypertension, Essential
Research Articles on ATP1b1
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Product Notes
The Human ATP1b1 atp1b1 (Catalog #AAA456837) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456837 ELISA Kit recognizes Human ATP1b1. It is sometimes possible for the material contained within the vial of "ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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