Human Elastase 3A (ELA3A) ELISA Kit | ELA3A elisa kit

Human Elastase 3A (ELA3A) ELISA Kit

Cat. Num. AAA455447

• Gene Names: CELA3A; ELA3; ELA3A
• Reactivity: Human
• Synonyms: Elastase 3A (ELA3A); Human Elastase 3A (ELA3A) ELISA Kit; CELA3A; ELA3; Chymotrypsin-Like Elastase Family Member 3A; Protease E; ELA3A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ELA3A. No significant cross-reactivity or interference between ELA3A and analogues was observed.
• Sequence Length: 270

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.113ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ELA3A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ELA3A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELA3A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ELA3A in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELA3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ELA3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ELA3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 236460050
• NCBI GeneID: 10136
• NCBI Accession #: NP_005738.4
• NCBI GenBank Nucleotide #: NM_005747.4
• UniProt Accession #: P09093; Q9BRW4; B1AQ53
• Molecular Weight: 29,489 Da
• NCBI Official Full Name: chymotrypsin-like elastase family member 3A preproprotein
• NCBI Official Synonym Full Names: chymotrypsin like elastase family member 3A
• NCBI Official Symbol: CELA3A
• NCBI Official Synonym Symbols: ELA3; ELA3A
• NCBI Protein Information: chymotrypsin-like elastase family member 3A
• UniProt Protein Name: Chymotrypsin-like elastase family member 3A
• UniProt Gene Name: CELA3A
• UniProt Synonym Gene Names: ELA3; ELA3A

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008]

Uniprot Description

Efficient protease with alanine specificity but only little elastolytic activity.