Human Elastase 3A (ELA3A) ELISA Kit | ELA3A elisa kit

Human Elastase 3A (ELA3A) ELISA Kit

Cat. Num. AAA2705411

• Gene Names: CELA3A; ELA3; ELA3A
• Reactivity: Human
• Synonyms: Elastase 3A (ELA3A); Human Elastase 3A (ELA3A) ELISA Kit; CELA3A; ELA3; Chymotrypsin-Like Elastase Family Member 3A; Protease E; ELA3A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Elastase 3A (ELA3A).; No significant cross-reactivity or interference between Elastase 3A (ELA3A) and analogues was observed.
• Sequence Length: 270

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 13.4pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastase 3A (ELA3A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastase 3A (ELA3A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELA3A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3A (ELA3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3A (ELA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3A (ELA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 3A (ELA3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119615407
• NCBI GeneID: 10136
• UniProt Accession #: P09093; Q9BRW4; B1AQ53
• Molecular Weight: 29,489 Da
• NCBI Official Full Name: elastase 3A, pancreatic, isoform CRA_b
• NCBI Official Synonym Full Names: chymotrypsin like elastase family member 3A
• NCBI Official Symbol: CELA3A
• NCBI Official Synonym Symbols: ELA3; ELA3A
• NCBI Protein Information: chymotrypsin-like elastase family member 3A
• UniProt Protein Name: Chymotrypsin-like elastase family member 3A
• UniProt Gene Name: CELA3A
• UniProt Synonym Gene Names: ELA3; ELA3A

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008]

Uniprot Description

Efficient protease with alanine specificity but only little elastolytic activity.