Human Ribonuclease A3 (RNASE3) ELISA Kit | RNASE3 elisa kit

Human Ribonuclease A3 (RNASE3) ELISA Kit

Cat. Num. AAA455305

• Gene Names: RNASE3; ECP; RAF1; RNS3
• Reactivity: Human
• Synonyms: Ribonuclease A3 (RNASE3); Human Ribonuclease A3 (RNASE3) ELISA Kit; Rnase-A3; RnaseA3; ECP; RNS3; Ribonuclease; RNase A Family 3; Eosinophil Cationic Protein; RNASE3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RNASE3. No significant cross-reactivity or interference between RNASE3 and analogues was observed.
• Sequence Length: 160

• Samples: Serum, plasma, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: < 28.43pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RNASE3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RNASE3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RNASE3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of RNASE3 in human serum, plasma, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNASE3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RNASE3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNASE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RNASE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45243507
• NCBI GeneID: 6037
• NCBI Accession #: NP_002926.2
• NCBI GenBank Nucleotide #: NM_002935.2
• UniProt Accession #: P12724; Q4VBC1; Q8WTP7; Q8WZ62; Q9GZN9
• Molecular Weight: 18,385 Da
• NCBI Official Full Name: eosinophil cationic protein
• NCBI Official Synonym Full Names: ribonuclease A family member 3
• NCBI Official Symbol: RNASE3
• NCBI Official Synonym Symbols: ECP; RAF1; RNS3
• NCBI Protein Information: eosinophil cationic protein
• UniProt Protein Name: Eosinophil cationic protein
• Protein Family: Eosinophil cationic protein
• UniProt Gene Name: RNASE3
• UniProt Synonym Gene Names: ECP; RNS3; ECP; RNase 3

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]

Uniprot Description

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.