Typical Testing Data/Standard Curve (for reference only)
Human Metastasis Associated In Colon Cancer 1 (MACC1) ELISA Kit | MACC1 elisa kit
Human Metastasis Associated In Colon Cancer 1 (MACC1) ELISA Kit
Gene Names
MACC1; 7A5; SH3BP4L
Reactivity
Human
Synonyms
Metastasis Associated In Colon Cancer 1 (MACC1); Human Metastasis Associated In Colon Cancer 1 (MACC1) ELISA Kit; IBP-7; IGF-binding protein 7; IGFBP-7; IGFBP-rP1; Insulin-like growth factor-binding protein 7; MAC25; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; PSF; TAF; MACC1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of MACC1. No significant cross-reactivity or interference between MACC1 and analogues was observed.
Sequence Length
852
Samples
Serum, plasma, tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.127ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MACC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MACC1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MACC1 elisa kit
Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MACC1 in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MACC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MACC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MACC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MACC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MACC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MACC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MACC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MACC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,639 Da
NCBI Official Full Name
metastasis-associated in colon cancer protein 1
NCBI Official Synonym Full Names
MACC1, MET transcriptional regulator
NCBI Official Symbol
MACC1
NCBI Official Synonym Symbols
7A5; SH3BP4L
NCBI Protein Information
metastasis-associated in colon cancer protein 1
UniProt Protein Name
Metastasis-associated in colon cancer protein 1
Protein Family
UniProt Gene Name
MACC1
NCBI Description
MACC1 is a key regulator of the hepatocyte growth factor (HGF; MIM 142409)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed 19098908]).[supplied by OMIM, Mar 2009]
Uniprot Description
Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.
Research Articles on MACC1
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Product Notes
The Human MACC1 macc1 (Catalog #AAA453881) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA453881 ELISA Kit recognizes Human MACC1. It is sometimes possible for the material contained within the vial of "Metastasis Associated In Colon Cancer 1 (MACC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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