Mouse Ki-67 Protein (Ki67P) ELISA Kit | Ki67P elisa kit

Mouse Ki-67 Protein (Ki67P) ELISA Kit

Cat. Num. AAA453642

• Gene Names: Mki67; Ki67; Ki-67; D630048A14Rik
• Reactivity: Mouse
• Synonyms: Ki-67 Protein (Ki67P); Mouse Ki-67 Protein (Ki67P) ELISA Kit; MKI67; KIA; Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; Ki67P elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ki67P. No significant cross-reactivity or interference between Ki67P and analogues was observed.
• Sequence Length: 3177

• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.108ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ki67P were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ki67P were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ki67P elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Ki67P in mouse tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ki67P. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Ki67P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ki67P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ki67P in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 169234624
• NCBI GeneID: 17345
• NCBI Accession #: NP_001074586.2
• NCBI GenBank Nucleotide #: NM_001081117.2
• UniProt Accession #: E9PVX6; Q61769; Q7TSF6
• Molecular Weight: 324,718 Da
• NCBI Official Full Name: proliferation marker protein Ki-67
• NCBI Official Synonym Full Names: antigen identified by monoclonal antibody Ki 67
• NCBI Official Symbol: Mki67
• NCBI Official Synonym Symbols: Ki67; Ki-67; D630048A14Rik
• NCBI Protein Information: proliferation marker protein Ki-67
• UniProt Protein Name: Proliferation marker protein Ki-67
• UniProt Gene Name: Mki67
• UniProt Synonym Gene Names: Antigen KI-67 homologCurated; Antigen Ki67 homologCurated

Uniprot Description

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.