Human CUB Domain Containing Protein 1 (CDCP1) ELISA Kit | CDCP1 elisa kit

Human CUB Domain Containing Protein 1 (CDCP1) ELISA Kit

Cat. Num. AAA452981

• Gene Names: CDCP1; CD318; TRASK; SIMA135
• Reactivity: Human
• Synonyms: CUB Domain Containing Protein 1 (CDCP1); Human CUB Domain Containing Protein 1 (CDCP1) ELISA Kit; F11R; JAM-A; JCAM; Junctional adhesion molecule A; PAM-1; Platelet adhesion molecule 1; Platelet F11 receptor; CDCP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CDCP1. No significant cross-reactivity or interference between CDCP1 and analogues was observed.
• Sequence Length: 343

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.127ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CDCP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CDCP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDCP1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDCP1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDCP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CDCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CDCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CDCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 30410805
• NCBI GeneID: 64866
• NCBI Accession #: NP_073753.3
• NCBI GenBank Nucleotide #: NM_022842.4
• UniProt Accession #: Q9H5V8; Q49UB4; Q6NT71; Q6U9Y2; Q8WU91; Q96QU7; Q9H676; Q9H8C2
• Molecular Weight: 37,818 Da
• NCBI Official Full Name: CUB domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: CUB domain containing protein 1
• NCBI Official Symbol: CDCP1
• NCBI Official Synonym Symbols: CD318; TRASK; SIMA135
• NCBI Protein Information: CUB domain-containing protein 1
• UniProt Protein Name: CUB domain-containing protein 1
• Protein Family: CUB domain-containing protein
• UniProt Gene Name: CDCP1
• UniProt Synonym Gene Names: TRASK; SIMA135

NCBI Description

This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.