Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit | Bcl2 elisa kit

Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

Cat. Num. AAA452319

• Gene Names: Bcl2; Bcl-2
• Reactivity: Rat
• Synonyms: B-Cell Leukemia/Lymphoma 2 (Bcl2); Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit; B-Cell CLL/Lymphoma 2; Bcl2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2. No significant cross-reactivity or interference between Bcl2 and analogues was observed.
• Sequence Length: 236

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: < 30.98pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Bcl2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Bcl2 in rat serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Bcl2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bcl2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8392974
• NCBI GeneID: 24224
• NCBI Accession #: NP_058689.1
• NCBI GenBank Nucleotide #: NM_016993.1
• UniProt Accession #: P49950; Q62837; Q64032
• Molecular Weight: 26,622 Da
• NCBI Official Full Name: apoptosis regulator Bcl-2
• NCBI Official Synonym Full Names: BCL2, apoptosis regulator
• NCBI Official Symbol: Bcl2
• NCBI Official Synonym Symbols: Bcl-2
• NCBI Protein Information: apoptosis regulator Bcl-2
• UniProt Protein Name: Apoptosis regulator Bcl-2
• Protein Family: Apoptosis regulator
• UniProt Gene Name: Bcl2
• UniProt Synonym Gene Names: Bcl-2

NCBI Description

an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD, Feb 2006]

Uniprot Description

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release ().