Mouse Fibroblast Growth Factor 7 (FGF7) ELISA Kit | FGF7 elisa kit

Mouse Fibroblast Growth Factor 7 (FGF7) ELISA Kit

Cat. Num. AAA452188

• Gene Names: Fgf7; Kgf
• Reactivity: Mouse
• Synonyms: Fibroblast Growth Factor 7 (FGF7); Mouse Fibroblast Growth Factor 7 (FGF7) ELISA Kit; KGF; HBGF7; Keratinocyte Growth Factor; Heparin-binding growth factor 7; FGF7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGF7. No significant cross-reactivity or interference between FGF7 and analogues was observed.
• Sequence Length: 194

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 34.55pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for FGF7 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FGF7 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGF7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FGF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FGF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FGF7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6679785
• NCBI GeneID: 14178
• NCBI Accession #: NP_032034.1
• NCBI GenBank Nucleotide #: NM_008008.4
• UniProt Accession #: P36363
• Molecular Weight: 22,347 Da
• NCBI Official Full Name: fibroblast growth factor 7
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: Fgf7
• NCBI Official Synonym Symbols: Kgf
• NCBI Protein Information: fibroblast growth factor 7
• UniProt Protein Name: Fibroblast growth factor 7
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf7
• UniProt Synonym Gene Names: Fgf-7; Kgf; FGF-7; HBGF-7; KGF

Uniprot Description

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation ().