Human Islet Amyloid Polypeptide (IAPP) ELISA Kit | IAPP elisa kit

Human Islet Amyloid Polypeptide (IAPP) ELISA Kit

Cat. Num. AAA450907

• Gene Names: IAPP; DAP; IAP
• Reactivity: Human
• Synonyms: Islet Amyloid Polypeptide (IAPP); Human Islet Amyloid Polypeptide (IAPP) ELISA Kit; IAP; DAP; Amylin; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide; IAPP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IAPP. No significant cross-reactivity or interference between IAPP and analogues was observed.
• Sequence Length: 89

• Samples: Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 7.81-500pg/mL
• Sensitivity: <2.26pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IAPP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IAPP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Related Product Information for IAPP elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IAPP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to IAPP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IAPP and unlabeled IAPP (Standards or samples) with the pre-coated antibody specific to IAPP. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IAPP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IAPP in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4557655
• NCBI GeneID: 3375
• NCBI Accession #: NP_000406.1
• NCBI GenBank Nucleotide #: NM_000415.2
• UniProt Accession #: P10997; Q0ZD87; Q14598
• Molecular Weight: 9,806 Da
• NCBI Official Full Name: islet amyloid polypeptide preproprotein
• NCBI Official Synonym Full Names: islet amyloid polypeptide
• NCBI Official Symbol: IAPP
• NCBI Official Synonym Symbols: DAP; IAP
• NCBI Protein Information: islet amyloid polypeptide
• UniProt Protein Name: Islet amyloid polypeptide
• Protein Family: Islet amyloid polypeptide
• UniProt Gene Name: IAPP
• UniProt Synonym Gene Names: DAP

NCBI Description

This gene encodes a member of the calcitonin family of peptide hormones. This hormone is released from pancreatic beta cells following food intake to regulate blood glucose levels and act as a satiation signal. Human patients with type 1 and advanced type 2 diabetes exhibit reduced levels of the encoded hormone in blood and pancreas. This protein also exhibits a bactericidal, antimicrobial activity. [provided by RefSeq, Jul 2016]

Uniprot Description

Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.