Human Ras Association Domain Containing Protein 1 (RASSF1) RTU ELISA Kit | RASSF1 rtu elisa kit

Human Ras Association Domain Containing Protein 1 (RASSF1) ELISA Kit

Cat. Num. AAA4503749

• Gene Names: RASSF1; 123F2; RDA32; NORE2A; RASSF1A; REH3P21
• Reactivity: Human
• Synonyms: Ras Association Domain Containing Protein 1 (RASSF1); Human Ras Association Domain Containing Protein 1 (RASSF1) ELISA Kit; 123F2; NORE2A; RASSF1A; RDA32; REH3P21; RASSF1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 189

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RASSF1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RASSF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RASSF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RASSF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RASSF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 332701155
• NCBI GeneID: 11186
• NCBI Accession #: NP_001193886.1
• NCBI GenBank Nucleotide #: NM_001206957.1
• UniProt Accession #: Q9NS23; O14571; O60539; O60710; Q0VGC6; Q5TZT2; Q9HB04; Q9HB18; Q9NS22; Q9UND4; Q9UND5; B7ZLL1
• Molecular Weight: 8,656 Da
• NCBI Official Full Name: ras association domain-containing protein 1 isoform B
• NCBI Official Synonym Full Names: Ras association domain family member 1
• NCBI Official Symbol: RASSF1
• NCBI Official Synonym Symbols: 123F2; RDA32; NORE2A; RASSF1A; REH3P21
• NCBI Protein Information: ras association domain-containing protein 1
• UniProt Protein Name: Ras association domain-containing protein 1
• Protein Family: Ras association domain-containing protein
• UniProt Gene Name: RASSF1

NCBI Description

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]

Uniprot Description

Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.