Rat Voltage Dependent Anion Channel Protein 1 (VDAC1) RTU ELISA Kit | VDAC1 rtu elisa kit

Rat Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit

Cat. Num. AAA4503278

• Gene Names: VDAC1; PORIN; VDAC-1
• Reactivity: Rat
• Synonyms: Voltage Dependent Anion Channel Protein 1 (VDAC1); Rat Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit; PORIN-31-HL; Porin 31HM; Plasmalemmal porin; Outer mitochondrial membrane protein porin 1; Voltage-dependent anion-selective channel protein 1; VDAC1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 283

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VDAC1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDAC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VDAC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VDAC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VDAC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507879
• NCBI GeneID: 7416
• NCBI Accession #: NP_003365.1
• NCBI GenBank Nucleotide #: NM_003374.2
• UniProt Accession #: P21796; Q5FVE7; Q9UIQ5; Q9UPL0; B3KVK4; D3DQ93
• Molecular Weight: 30,773 Da
• NCBI Official Full Name: voltage-dependent anion-selective channel protein 1
• NCBI Official Synonym Full Names: voltage dependent anion channel 1
• NCBI Official Symbol: VDAC1
• NCBI Official Synonym Symbols: PORIN; VDAC-1
• NCBI Protein Information: voltage-dependent anion-selective channel protein 1
• UniProt Protein Name: Voltage-dependent anion-selective channel protein 1
• Protein Family: Voltage-dependent anion-selective channel protein
• UniProt Gene Name: VDAC1
• UniProt Synonym Gene Names: VDAC; VDAC-1; hVDAC1

NCBI Description

This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010]

Uniprot Description

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756).