Rat Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) RTU ELISA Kit | TRPM4 rtu elisa kit

Rat Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit

Cat. Num. AAA4503122

• Gene Names: Trpm4; Mls2s; LTrpC-4
• Reactivity: Rat
• Synonyms: Transient Receptor Potential Cation Channel Subfamily M; Member 4 (TRPM4); Rat Transient Receptor Potential Cation Channel Subfamily M; Member 4 (TRPM4) ELISA Kit; TRPM4B; Melastatin-4; Calcium-activated non-selective cation channel 1; Long transient receptor potential channel 4; TRPM4 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 1208

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRPM4 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRPM4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TRPM4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TRPM4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TRPM4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 209977101
• NCBI GeneID: 171143
• NCBI Accession #: NP_001129701.1
• NCBI GenBank Nucleotide #: NM_001136229.1
• UniProt Accession #: Q9ESQ5
• Molecular Weight: 115,277 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 4
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily M, member 4
• NCBI Official Symbol: Trpm4
• NCBI Official Synonym Symbols: Mls2s; LTrpC-4
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 4
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 4
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: Trpm4
• UniProt Synonym Gene Names: Ltrpc4; LTrpC-4; LTrpC4

NCBI Description

member of a family of calcium permeable channels [RGD, Feb 2006]

Uniprot Description

Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca2+, it is impermeable to it. Mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca2+ overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Essential for the migration but not the maturation of dendritic cells ().