Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit | TRPM4 elisa kit

Mouse Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit

Cat. Num. AAA2705922

• Gene Names: TRPM4; LTrpC4; PFHB1B; TRPM4B; hTRPM4
• Reactivity: Mouse
• Synonyms: Transient Receptor Potential Cation Channel Subfamily M; Member 4 (TRPM4); Mouse Transient Receptor Potential Cation Channel Subfamily M; Member 4 (TRPM4) ELISA Kit; TRPM4B; Melastatin-4; Calcium-activated non-selective cation channel 1; Long transient receptor potential channel 4; TRPM4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4).; No significant cross-reactivity or interference between Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) and analogues was observed.
• Sequence Length: 1,069

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.048ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRPM4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 304766677
• NCBI GeneID: 54795
• NCBI Accession #: NP_001182156.1
• NCBI GenBank Nucleotide #: NM_001195227.1
• UniProt Accession #: Q8TD43; Q7Z5D9; Q96L84; Q9NXV1; A2RU25
• Molecular Weight: 118,630 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 4 isoform 2
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 4
• NCBI Official Symbol: TRPM4
• NCBI Official Synonym Symbols: LTrpC4; PFHB1B; TRPM4B; hTRPM4
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 4
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 4
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPM4
• UniProt Synonym Gene Names: LTRPC4; hTRPM4; LTrpC-4; LTrpC4

NCBI Description

The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. [provided by RefSeq, Mar 2016]

Uniprot Description

Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca2+, it is impermeable to it. Mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca2+ overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway.