Mouse Transglutaminase 3, Epidermal (TGM3) RTU ELISA Kit | TGM3 rtu elisa kit

Mouse Transglutaminase 3, Epidermal (TGM3) ELISA Kit

Cat. Num. AAA4502843

• Gene Names: Tgm3; we; TGE; TG E; TG(E); TGase E; TGase-3; AI893889
• Reactivity: Mouse
• Synonyms: Transglutaminase 3; Epidermal (TGM3); Mouse Transglutaminase 3; Epidermal (TGM3) ELISA Kit; TGE; Egt; Protein-Glutamine Gamma-Glutamyltransferase E; Transglutaminase E; TGM3 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 693

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.255ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGM3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGM3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TGM3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGM3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 124001551
• NCBI GeneID: 21818
• NCBI Accession #: NP_033400.2
• NCBI GenBank Nucleotide #: NM_009374.3
• UniProt Accession #: Q08189; A1L343; A2ANC3
• Molecular Weight: 77,309 Da
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase E
• NCBI Official Synonym Full Names: transglutaminase 3, E polypeptide
• NCBI Official Symbol: Tgm3
• NCBI Official Synonym Symbols: we; TGE; TG E; TG(E); TGase E; TGase-3; AI893889
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase E
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase E
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: Tgm3
• UniProt Synonym Gene Names: Tgase3; TG(E); TGE; TGase E; TGase-3

Uniprot Description

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.