Human Small Heterodimer Partner (SHP) RTU ELISA Kit | SHP rtu elisa kit

Human Small Heterodimer Partner (SHP) ELISA Kit

Cat. Num. AAA4502486

• Gene Names: NR0B2; SHP; SHP1
• Reactivity: Human
• Synonyms: Small Heterodimer Partner (SHP); Human Small Heterodimer Partner (SHP) ELISA Kit; NR0B2; SHP1; Nuclear Receptor Subfamily 0; Group B; Member 2; Orphan nuclear receptor SHP; SHP rtu elisa kit

• Reactivity: Human
• Sequence Length: 257

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SHP rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SHP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SHP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SHP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13259503
• NCBI GeneID: 8431
• NCBI Accession #: NP_068804.1
• NCBI GenBank Nucleotide #: NM_021969.2
• UniProt Accession #: Q15466; Q5QP36; F1D8P5
• Molecular Weight: 28,058 Da
• NCBI Official Full Name: nuclear receptor subfamily 0 group B member 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0 group B member 2
• NCBI Official Symbol: NR0B2
• NCBI Official Synonym Symbols: SHP; SHP1
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 2
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 2
• Protein Family: Head decoration protein
• UniProt Gene Name: NR0B2
• UniProt Synonym Gene Names: SHP

NCBI Description

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1.