Typical Testing Data/Standard Curve (for reference only)
Mouse Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit | CXCR4 elisa kit
Mouse Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit
Gene Names
Cxcr4; CD184; LESTR; Sdf1r; Cmkar4; PB-CKR; b2b220Clo; PBSF/SDF-1
Reactivity
Mouse
Synonyms
Chemokine C-X-C-Motif Receptor 4 (CXCR4); Mouse Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit; CD184; Fusin; NPYR; HSY3RR; LAP3; LCR1; LESTR; NPY3R; NPYRL; WHIM; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; CXCR4 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of CXCR4. No significant cross-reactivity or interference between CXCR4 and analogues was observed.
Sequence Length
359
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
27.2pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CXCR4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CXCR4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCR4 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CXCR4 in mouse tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXCR4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CXCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CXCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CXCR4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXCR4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CXCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CXCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CXCR4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,240 Da
NCBI Official Full Name
C-X-C chemokine receptor type 4
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 4
NCBI Official Symbol
Cxcr4
NCBI Official Synonym Symbols
CD184; LESTR; Sdf1r; Cmkar4; PB-CKR; b2b220Clo; PBSF/SDF-1
NCBI Protein Information
C-X-C chemokine receptor type 4
UniProt Protein Name
C-X-C chemokine receptor type 4
Protein Family
UniProt Gene Name
Cxcr4
UniProt Synonym Gene Names
Cmkar4; Lestr; Sdf1r; CXC-R4; CXCR-4; LESTR; PB-CKR; SDF-1 receptor
Uniprot Description
Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (). Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival.
Research Articles on CXCR4
1. crosstalk between astrocytic CXCL12 and microglial CXCR4 in the pathogenesis of neuropathic pain.
Similar Products
Product Notes
The Mouse CXCR4 cxcr4 (Catalog #AAA450169) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA450169 ELISA Kit recognizes Mouse CXCR4. It is sometimes possible for the material contained within the vial of "Chemokine C-X-C-Motif Receptor 4 (CXCR4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
