Mouse anti-Human NFATC1 Monoclonal Antibody | anti-NFATC1 antibody

Mouse Monoclonal [clone AT1C3] (IgG2a,k) to Human NFATC1

Cat. Num. AAA245376

• Gene Names: NFATC1; NFAT2; NFATc; NF-ATC
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein G Purified
• Synonyms: NFATC1; Monoclonal Antibody; Mouse Monoclonal [clone AT1C3] (IgG2a; k) to Human NFATC1; Anti-NFATC1 Antibody (aa428-716; clone AT1C3) IHC-plus; NFATc; NFAT2; NF-ATC; NF-ATC transcription factor; NF-ATc1; Human NFATC1; anti-NFATC1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: AT1C3
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 825

• Applicable Applications for anti-NFATC1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 ug/ml), WB (1:1000 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human NFATc1 (428-716aa) purified from E Coli. NCBI Accession No.: NP_765978.
• Immunogen Type: Recombinant protein
• Immunogen: NFATC1 antibody was raised against recombinant human NFATc1 (428-716aa) purified from E Coli. NCBI Accession No.: NP_765978.
• Antigen Modification: aa428-716
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-NFATC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 19923350
• NCBI GeneID: 4772
• NCBI Accession #: NP_006153.2
• NCBI GenBank Nucleotide #: NM_006162.4
• UniProt Accession #: O95644; Q12865; Q15793; Q2M1S3; B5B2M4; B5B2M5; B5B2M6; B5B2M7; B5B2M8; B5B2M9; B5B2N1
• Molecular Weight: 38,626 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 1 isoform B
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
• NCBI Official Symbol: NFATC1
• NCBI Official Synonym Symbols: NFAT2; NFATc; NF-ATC
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 1; NFAT transcription complex cytosolic component
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: NFATC1
• UniProt Synonym Gene Names: NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc
• UniProt Entry Name: NFAC1_HUMAN

NCBI Description

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

Uniprot Description

NFAT2: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Six splice variant isoforms have been identified. Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; mitogen-activated protein kinase p38 binding; FK506 binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; calcium ion transport; heart development; positive regulation of transcription, DNA-dependent; epithelial to mesenchymal transition; innate immune response; positive regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; G1/S transition of mitotic cell cycle