Rat Linker For Activation Of T-Cell (LAT) RTU ELISA Kit | LAT rtu elisa kit

Rat Linker For Activation Of T-Cell (LAT) ELISA Kit

Cat. Num. AAA4500694

• Reactivity: Rat
• Synonyms: Linker For Activation Of T-Cell (LAT); Rat Linker For Activation Of T-Cell (LAT) ELISA Kit; LAT1; Pp36; Linker Of Activated T Cells; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; LAT rtu elisa kit

• Reactivity: Rat
• Sequence Length: 241

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.036ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAT rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13540669
• NCBI GeneID: 81511
• NCBI Accession #: NP_110480.1
• NCBI GenBank Nucleotide #: NM_030853.1
• UniProt Accession #: O70601
• Molecular Weight: 26,193 Da
• NCBI Official Full Name: linker for activation of T-cells family member 1
• NCBI Official Synonym Full Names: linker for activation of T cells
• NCBI Official Symbol: Lat
• NCBI Protein Information: linker for activation of T-cells family member 1
• UniProt Protein Name: Linker for activation of T-cells family member 1
• Protein Family: L-lysine-epsilon aminotransferase
• UniProt Gene Name: Lat
• UniProt Synonym Gene Names: pp36

NCBI Description

an adaptor protein; involved in signalling after T-cell activation [RGD, Feb 2006]

Uniprot Description

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules ().